Microbial analysis of subglacial samples drilling at Dome Fuji, Antarctica

• Project/Area Number: 24710020
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Environmental dynamic analysis
• Research Institution: 大学共同利用機関法人情報・システム研究機構(新領域融合研究センター及びライフサイ
• Principal Investigator: SEGAWA Takahiro 大学共同利用機関法人情報・システム研究機構(新領域融合研究センター及びライフサイ, 大学共同利用機関等の部局等, 助教 (90425835)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: アイスコア / 微生物 / 極限環境 / 古代試料 / 南極 / 極限微生物

Research Abstract

The ice cores from Antarctica could preserve microorganisms in the ancient atmosphere trapped in the snow and ice from the ice sheet. Subglacial environment are thought to be contained ancient ecosystems. We examine microorganisms in the ice core samples near the bedrock by molecular DNA techniques. We extracted the inner part of ice core samples using the melting device special designed for biological analysis. Small DNA fragments were extracted from ice samples and sequenced using Illumina Hiseq and Miseq sequencers. We carried out taxonomic and functional binning of the metagenomic DNA, and attempted to reconstruct the organisms. Our data support that microorganism have existed in this subglacial environment.

Research Products

• [Journal Article] 雪氷中のバクテリアが語るもの (2014)
  • Author(s): 瀬川高弘,牛田一成,幸島司郎
  • Journal Title: 雪氷 Volume: 76 Pages: 59-67
  • Peer Reviewed
• [Journal Article] Distribution of antibiotic resistance genes in glacier environments (2013)
  • Author(s): Takahiro Segawa, Nozomu Takeuchi, Andres Rivera, Akinori Yamada, Yoshitaka Yoshimura, Gonzalo Barcaza, Kunio Shinbori, Hideaki Motoyama, Shiro Kohshima and Kazunari Ushida
  • Journal Title: Environmental Microbiology Report Volume: 5 (1) Pages: 127-134
  • Peer Reviewed
• [Journal Article] Recent melting status at the upstream part of the Gangjoe La Glacier, Bhutan Himalayas, determined by pollen analysis of an ice core (2013)
  • Author(s): Fumio NAKAZAWA, Takahiro SEGAWA, Shiro KOHSHIMA, Lobzang GYENDEN, Toshiyuki FUJIKI and Karma TOEB.
  • Journal Title: Bulletin of Glaciological Research Volume: 31 Pages: 1-7
  • NAID: 130004553905
  • Peer Reviewed
• [Journal Article] Simultaneous Quantification of Multiple Food and Waterborne Pathogens by Use of Microfluidic Quantitative PCR (2013)
  • Author(s): Ishii, S.
  • Journal Title: Applied and Environmental Microbiology Volume: 79 Issue: 9 Pages: 2891-2898
  • DOI: 10.1128/aem.00205-13
  • Peer Reviewed
• [Journal Article] Recent melting status at the upstream part of the Gangjoe La Glacier, Bhutan Himalayas, determined by pollen analysis of an ice core. (2013)
  • Author(s): Fumio NAKAZAWA, Takahiro SEGAWA, Shiro KOHSHIMA, Lobzang GYENDEN, Toshiyuki FUJIKI and Karma TOEB
  • Journal Title: Bulletin of Glaciological Research Volume: 31 Pages: 1-4
  • NAID: 130004553905
  • Peer Reviewed
• [Journal Article] Distribution of antibiotic resistance genes in glacier environments (2012)
  • Author(s): Segawa, T., N. Takeuchi, A. Rivera, A. Yamada, Y. Yoshimura G. Barcaza, K. Shinbori, H.i. Motoyama, S. Kohshima, and K. Ushida
  • Journal Title: Environmental Microbiology Reports 5 Volume: 5 Issue: 1 Pages: 127-134
  • DOI: 10.1111/1758-2229.12011
  • Peer Reviewed
• [Presentation] Ancient DNA analysis method (2014)
  • Author(s): Takahiro Segawa
  • Organizer: International Workshop on Methods for Biodiversity Research
  • Place of Presentation: 中国、上海
  • Invited
• [Presentation] Reconstructions of past flora using DNA analysis from Greenland icecore samples (2013)
  • Author(s): Takahiro Segawa, Shinji Kondo and Hideaki Motoyama
  • Organizer: The 4th Symposium on Polar Science
• [Presentation] 南極氷床底面から検出された微生物解析 (2013)
  • Author(s): 近藤伸二,瀬川高弘,本山秀明
  • Organizer: 極域科学シンポジウム
• [Presentation] Nitrogen cycle in cryoconites : naturally-occurring nitrification-denitrification granules on a glacier in Central Asia (2013)
  • Author(s): Takahiro Segawa, Satoshi Ishii, Nobuto Ohte, Zhongqin Li, Yuichi Hongoh, Nozomu Takeuchi
  • Organizer: 3rd International conference on Nitrification
• [Presentation] キルギス・グレゴリア氷河のアイスコア試料を用いたメタゲノム解析による古環境復元 (2013)
  • Author(s): 瀬川高弘,近藤伸二,竹内望
  • Organizer: 地球惑星科学連合大会
• [Presentation] Nitrogen cycle in cryoconites: naturally-occurring nitrification-denitrification granules on a glacier in Central Asia (2013)
  • Author(s): Takahiro Segawa, Satoshi Ishii, Nobuto Ohte, Zhongqin Li, Yuichi Hongoh, Nozomu Takeuchi.
  • Organizer: 3rd International conference on Nitrification
  • Place of Presentation: 東京
• [Presentation] Reconstructions of past flora using DNA analysis from Greenland icecore samples (2013)
  • Author(s): Takahiro Segawa, Shinji Kondo and Hideaki Motoyama
  • Organizer: 極域科学シンポジウム
  • Place of Presentation: 東京
• [Presentation] 極氷床底面から検出された微生物解析 (2013)
  • Author(s): 近藤伸二、瀬川高弘、本山秀明
  • Organizer: 極域科学シンポジウム
  • Place of Presentation: 東京
• [Presentation] キルギス・グレゴリア氷河のアイスコア試料を用いたメタゲノム解析による古環境復元 (2013)
  • Author(s): 瀬川 高弘、近藤伸二、竹内 望
  • Organizer: 地球惑星科学連合大会
  • Place of Presentation: 千葉
• [Presentation] Simultaneous Quantification of Multiple Food and Waterborne Pathogens by Use of Microfluidic Quantitative PCR (2013)
  • Author(s): S. Ishii, T. Segawa, S. Okabe
  • Organizer: ASM General Meeting
  • Place of Presentation: 米国、デンバー
• [Presentation] キルギス・グレゴリア氷河のアイスコア試料を用いたメタゲノム解析による古環境復元 (2013)
  • Author(s): 瀬川 高弘、近藤伸二、竹内 望
  • Organizer: 地球惑星科学連合大会
  • Place of Presentation: 幕張メッセ
• [Presentation] 南米パタゴニア氷原チンダル氷河裸氷域表面の近年の変化 (2013)
  • Author(s): 藤澤 雄太、竹内望、幸島司郎、瀬川高弘、村上匠
  • Organizer: 地球惑星科学連合大会
  • Place of Presentation: 幕張メッセ
• [Presentation] 気球による成層圏微生物捕集実験 (2013)
  • Author(s): 大野 宗祐、石橋 高、山岸 明彦、河口 優子、仁田原 翔太、瀬川 高弘、所源亮、山内 一也、石川 裕子、三宅 範宗、小林 正規、松井孝典
  • Organizer: 地球惑星科学連合大会
  • Place of Presentation: 幕張メッセ
• [Presentation] 氷床アイスコア中の微生物解析 (2012)
  • Author(s): 瀬川高弘,近藤伸二,本山秀明
  • Organizer: 日本細菌学会細菌学若手コロッセウム
  • Year and Date: 2012-08-08
• [Presentation] Distribution of cyanobacteria in glacier environment (2012)
  • Author(s): Takahiro Segawa
  • Organizer: International Workshop on Glacial Ecology
  • Year and Date: 2012-04-09
• [Presentation] Impact of microbial communities on nitrogen cycles on Urumqi Glacier No.1 in China (2012)
  • Author(s): Takahiro Segawa, Satoshi Ishii, Dylan Bodington, Yuichi Hongoh, Fumito Maruyama, Nozomu Takeuchi
  • Organizer: AGU 2012 Fall Meeting
• [Presentation] Impact of microbial communities on nitrogen cycles on Urumqi Glacier No.1 in China, (2012)
  • Author(s): Takahiro Segawa; Satoshi Ishii; Dylan Bodington; Yuichi Hongoh; Fumito Maruyama; Nozomu Takeuchi
  • Organizer: AGU 2012 Fall Meeting
  • Place of Presentation: アメリカ、サンフランシスコ
• [Presentation] Distribution of cyanobacteria in glacier environment, International Workshop on Glacial Ecology (2012)
  • Author(s): Takahiro Segawa
  • Organizer: International Workshop on Glacial Ecology,
  • Place of Presentation: 千葉大学
• [Presentation] 氷床アイスコア中の微生物解析 (2012)
  • Author(s): 瀬川高弘，近藤伸二，本山秀明
  • Organizer: 日本細菌学会細菌学若手コロッセウム
  • Place of Presentation: 東京都八王子市 八王子セミナーハウス
• [Book] 極限環境生物の産業展開,「第5章アイスコア中の微生物解析」 (2012)
  • Author(s): 瀬川高弘
  • Publisher: シーエムシー出版
• [Book] 低温科学、氷河やアイスコア中のバクテリアに関する研究 (2012)
  • Author(s): 瀬川高弘
  • Total Pages: 8
  • Publisher: 北海道大学 低温科学研究所
• [Book] 極限環境生物の産業展開，「第5章 アイスコア中の微生物解析」，分担 (2012)
  • Author(s): 瀬川高弘
  • Total Pages: 8
  • Publisher: シーエムシー出版