| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
It is largely unknown whether modification of nucleotides by radiation contributes to its biological effects. In the present study, we comprehensively analyzed modification of purine nucleosides caused by X-ray irradiation. Using HPLC-PDA and LC-MS/MS systems, various forms of modified purine nucleosides in free purine nucleosides, DNA and RNA exposed to X-ray were identified in quantitative manners, and some of them were confirmed to be formed in cellular nucleotide pools after X-ray irradiation. We found that various modified nucleosides are generated in free purine nucleosides, DNA and RNA by X-ray irradiation in a dose dependent manner and free purine nucleosides are most susceptible to modification by X-ray irradiation among those examined. In addition, we indicated that short-term memory is affected by cranial irradiation in mice.
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