Research on the mechanism of radiation-induced brain dysfunction and the defense mechanism
| Project/Area Number |
24710060
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Kyushu University |
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Principal Investigator |
AKIMOTO Yoriko 九州大学, 生体防御医学研究所, 特別研究員 (50613254)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | X線 / 修飾ヌクレオシド / ラジカル / 放射線生物影響 / 修飾ヌクレオチド / UV / 放射線 / ヌクレオチドプール |
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| Outline of Final Research Achievements |
It is largely unknown whether modification of nucleotides by radiation contributes to its biological effects. In the present study, we comprehensively analyzed modification of purine nucleosides caused by X-ray irradiation. Using HPLC-PDA and LC-MS/MS systems, various forms of modified purine nucleosides in free purine nucleosides, DNA and RNA exposed to X-ray were identified in quantitative manners, and some of them were confirmed to be formed in cellular nucleotide pools after X-ray irradiation. We found that various modified nucleosides are generated in free purine nucleosides, DNA and RNA by X-ray irradiation in a dose dependent manner and free purine nucleosides are most susceptible to modification by X-ray irradiation among those examined. In addition, we indicated that short-term memory is affected by cranial irradiation in mice.
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Report
(5 results)
Research Products
(7 results)
