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Analysis of cell death and repair pathway choice of DNA double-strand breaks caused by anti-cancer drug

Research Project

Project/Area Number 24710061
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Risk sciences of radiation/Chemicals
Research InstitutionKanazawa Medical University

Principal Investigator

Sakasai Ryo  金沢医科大学, 医学部, 助教 (10549950)

Research Collaborator SHIBATA Atsushi  群馬大学, 先端科学研究指導者育成ユニット, 助教 (30707633)
Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
KeywordsDNA二本鎖切断 / 非相同末端連結 / 相同組換え / カンプトテシン / ユビキチン
Outline of Final Research Achievements

DNA double-strand breaks (DSBs) are severe DNA damage that can be caused by anti-cancer drug to kill cancer cells. Camptothecin (CPT) causes DSB via DNA replication in growing cells. However, cellular responses to DNA replication-mediated DSB (RM-DSB) had not been revealed. In this study, we analyzed regulatory mechanisms of RM-DSB repair pathway and discovered that UbcH5c, a E2 ubiquitin-conjugating enzyme, is involved in NHEJ pathway of RM-DSB repair leading to chromosomal aberration.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (6 results)

All 2015 2014 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (3 results) (of which Int'l Joint Research: 1 results) Remarks (2 results)

  • [Journal Article] The distinctive cellular responses to DNA strand breaks caused by a DNA topoisomerase I poison in conjunction with DNA replication and RNA transcription2015

    • Author(s)
      Sakasai R, Iwabuchi K.
    • Journal Title

      Genes & Genetic Systems

      Volume: 90 Issue: 4 Pages: 187-194

    • DOI

      10.1266/ggs.15-00023

    • NAID

      130005118395

    • ISSN
      1341-7568, 1880-5779
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Presentation] Ubiquitin-dependent activation of DNA-PKcs leads to chromosomal aberration in response to one-ended DNA double strand breaks2015

    • Author(s)
      Ryo Sakasai, Yumi Sunatani, Tadashi Matsui, Mitsumasa Hashimoto, Kuniyoshi Iwabuchi
    • Organizer
      ICRR2015
    • Place of Presentation
      京都国際会館(京都府京都市)
    • Year and Date
      2015-05-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Ubiquitin-dependent activation of DNA-PKcs in response to DNA replication-mediated DNA double strand breaks2014

    • Author(s)
      Ryo Sakasai, Yumi Sunatani, Tadashi Matsui, Mitsumasa Hashimoto, Kuniyoshi Iwabuchi
    • Organizer
      The 9th 3R Symposium
    • Place of Presentation
      御殿場高原ホテル時之栖(静岡県御殿場市)
    • Year and Date
      2014-11-17 – 2014-11-21
    • Related Report
      2014 Research-status Report
  • [Presentation] ユビキチン化を介したDNA-PKcs活性化機構2014

    • Author(s)
      逆井良、松井理、砂谷優実、橋本光正、岩淵邦芳
    • Organizer
      日本放射線影響学会第57回大会
    • Place of Presentation
      かごしま県民交流センター(鹿児島県鹿児島市)
    • Year and Date
      2014-10-01 – 2014-10-03
    • Related Report
      2014 Research-status Report
  • [Remarks] Iwabuchi Lab 金沢医科大学 生化学I

    • URL

      http://kmu-bc1.jimdo.com

    • Related Report
      2015 Annual Research Report
  • [Remarks] Iwabuchi Lab-金沢医科大学 生化学I

    • URL

      http://kmu-bc1.jimdo.com

    • Related Report
      2014 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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