Radiosensitivity and Radiation-Induced Autophagy in Thyroid Follicular Epithelial Cells of Immature and Adult Rats
| Project/Area Number |
24710062
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 放射線 / ラット / 甲状腺濾胞上皮 / 細胞死 / オートファジー / 年齢依存性 |
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| Research Abstract |
Exposure to ionizing radiation (IR), especially during childhood, is a well-known risk factor for thyroid cancers. To evaluate the radiosensitivity of different age rat thyroid follicular epithelial cells, we analyzed the histological alterations, induction of apoptosis, cell proliferation, expression of DNA damage response molecules, and induction of autophagy in immature (4 W) and adult (8 M) rat thyroid glands up to 72 h after 8Gy X-ray irradiation.
IR-induced apoptosis was not detected, and phosphorylated p53 at Ser15 expression and 53BP1 nuclear foci were increased irrespective of age. The vacuolated alteration of the cytoplasm in thyroid follicular cells was observed in 4W. The Ki67-positive cells in 4W were significantly decreased after irradiation. The autophagosomes were observed in 4W cells but not 8M. The expression of LC3-II and p62, and the genes regulated autophagy was increased in 4W but not 8M. IR induced autophagy in immature but not in adult rat thyroid.
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Report
(3 results)
Research Products
(20 results)
