Elucidation of the molecular mechanism of chromosome translocation formation/suppression after exposure to ionizing radiation
| Project/Area Number |
24710063
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Nagasaki University (2013-2014)
Central Research Institute of Electric Power Industry (2012) |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 染色体転座 / DNA二本鎖切断 / フォーカス / 会合 / Ku80 / DNA-PKcs / ATM / 53BP1 / 放射線 / 近接 / DNA-PK |
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| Outline of Final Research Achievements |
Chromosome translocation is a clinically relevant chromosome aberration since it can cause leukemia, solid cancer, or other diseases, yet the molecular mechanism for translocation formation/suppression is unknown. In the present study, we identified factors affecting interaction between multiple DNA double-strand breaks (DSBs), that is prerequisite for translocation formation. We visualized DSBs by “foci” of a DNA repair factor accumulating at DSB sites, and examined frequency of DSB interaction. We identify Ku80, DNA-PKcs, and ATM as factors suppressing DSB interaction, and we also find that 53BP1 promotes DSB interaction and thereby facilitates translocation formation.
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Report
(4 results)
Research Products
(7 results)
