Evaluation of the mechanism for low sperm number and quality by decabrominated diphenyl ether

• Project/Area Number: 24710069
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Chiba University
• Principal Investigator: MIYASO Hidenobu 千葉大学, 予防医学センター, 特任助教 (80422252)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 臭素化系難燃剤 / 内分泌かく乱物質 / 雄性生殖 / 精子形成障害 / 選択的スプラインシング

Outline of Final Research Achievements

Principal investigator have clarified that exposure to decabrominated diphenyl ether (decaBDE) cause decreased sperm number in mice. In this study, principal investigator evaluated about the detailed mechanism for such toxicity. Results showed that neonatal exposure to decaBDE causes 1) decreased testosterone level in sera, 2) decreased transcript levels of androgen receptor and thyroid hormone receptor in testes. In addition, data in this study showed that 3) decaBDE affects the ratio of splicing products of thyroid hormone receptors in Sertoli cells. Such data elucidated one of the mechanisms to cause male reproductive toxicity by exposure to decaBDE.

Research Products

• [Journal Article] Early postnatal exposure to a low dose of decabromodiphenyl ether affects expression of androgen and thyroid hormone receptor-alpha and its splicing variants in mouse Sertoli cells (2014)
  • Author(s): Hidenobu Miyaso, Noriko Nakamura, Munekazu Naito, Shuichi Hirai, Yoshiharu Matsuno, Masahiro Itoh, Chisato Mori
  • Journal Title: PLoS One Volume: Dec 5;9(12) Issue: 12 Pages: e114487-e114487
  • DOI: 10.1371/journal.pone.0114487
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Changes in expression levels of oxidative stress-related genes in mouse epididymides by neonatal exposure to low-dose decabromodiphenyl ether (2014)
  • Author(s): Makoto Nakamoto, Hidenobu Miyaso, Masatoshi Komiyama, Yoshiharu Matsuno, Chisato Mori
  • Journal Title: Reprod Med Biol Volume: 13 Issue: 3 Pages: 127-134
  • DOI: 10.1007/s12522-013-0173-x
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Postnatal exposure to low-dose decabromodiphenyl ether adversely affects mouse testes by increasing thyrosine phosphorylation level of cortactin (2012)
  • Author(s): Miyaso H, Nakamura N, Matsuno Y, Kawashiro Y, Komiyama M, Mori C.
  • Journal Title: The Journal of Toxicological Sciences Volume: 37(5) Pages: 987-999
  • NAID: 10030876492
  • Peer Reviewed
• [Presentation] Neonatal exposure to decabromodiphenyl ether changes transcript levels of thyroid hormone receptor α and it alternative splicing variant in mouse Sertoli cells (2015)
  • Author(s): Hidenobu Miyaso, Noriko Nakamura, Yoshiharu Matsuno, Chisato Mori
  • Organizer: Society of Toxicology
  • Place of Presentation: Sandiego, California (USA)
  • Year and Date: 2015-03-22 – 2015-03-26
• [Presentation] Effect of neonatal exposure to decabromodiphenyl ether on transcript levels of thyroid hormone receptor α and androgen receptor in mouse Sertoli cells (2014)
  • Author(s): Hidenobu Miyaso, Noriko Nakamura, Yoshiharu Matsuno and Chisato Mori
  • Organizer: Ｓｏｃｉｅｔｙ ｏｆ Ｔｏｘｉｃｏｌｏｇｙ
  • Place of Presentation: Phoenix, Arizona (USA)
• [Presentation] Effect of exposure to decabromodiphenyl ether on alternative splicing of thyroid hormone receptor α transcript in mouse testis (2013)
  • Author(s): Hidenobu Miyaso, Masatoshi Komiyama, Yoshiharu Matsuno, and Chisato Mori
  • Organizer: 第118回日本解剖学会
  • Place of Presentation: 高松 香川
• [Presentation] Effect of neonatal exposure to decabromodiphenyl ether on transcript levels of splicing factors in mouse testes (2013)
  • Author(s): H. Miyaso, N. Nakamura, Y. Matsuno, M. Komiyama, and C. Mori
  • Organizer: 52th Society of Toxicology
  • Place of Presentation: San Antonio, Texas, USA
• [Presentation] Effect of postnatal exposure to decabromodiphenyl ether on expression of splicing variants of thyroid hormone α transcript and its splicing factors in mouse testes (2012)
  • Author(s): H. Miyaso, M. Komiyama, Y. Matsuno and C. Mori
  • Organizer: 第15回環境ホルモン学会
  • Place of Presentation: 東京大学 東京
• [Presentation] New observations concerning exposure to decabromodiphenyl ether on mouse testes (2012)
  • Author(s): H. Miyaso, N. Nakamura, Y. Matsuno, M. Komiyama, and C. Mori
  • Organizer: 第27回日本生殖免疫学会
  • Place of Presentation: 大阪医科大学 大阪
• [Presentation] Effect of neonatal exposure to decabromodiphenyl ether on serum thyroid hormone levels and thyroid hormone receptor alpha transcript level in mouse testes (2012)
  • Author(s): H. Miyaso, N. Nakamura, Y Matsuno, Y. Kawashiro, M. Nakamoto, M. Komiyama and C. Mori
  • Organizer: 15th International Congress on Hormonal Steroids and Hormones & Cancer
  • Place of Presentation: Ishikawa Ongakudo, Kanazawa, Japan