Investigation of endocrine-disrupting chemical effect for developmental process of organism using novel critical-window exposure method.
| Project/Area Number |
24710072
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Ariake National College of Technology |
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Principal Investigator |
YAMAGUCHI akemi 有明工業高等専門学校, その他部局等, 技術専門職員 (90399262)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 生物・生体工学 / 電力工学 / 発生・分化 / マイクロアレイ / 初期発生影響評価 / ビスフェノールA / エストロゲン / ビスフェノールS / 該当なし |
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| Outline of Final Research Achievements |
We developed novel material incorporation technic into medaka fertilized eggs using high voltage pulse systems. We assessed the effects of estradiol-17β (E2), bisphenol A (BPA) or BPA relative compounds such as bisphenol S (BPS) for medaka eggs by the incorporation technic and observed by microscope. It was observed that embryonic malformation and abnormal formation of bone, hart, blood vessel and hemocyte chemicals dose-dependently. In addition, we carried out DNA microarray analysis of medaka eggs treated with E2, BPA, BPA relative compounds, and detected variation of each chemicals specific genes expressions. It is suggested that these genes relation to morphological abnormality induced by each chemicals treatment.
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Report
(4 results)
Research Products
(10 results)
