| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
We developed novel material incorporation technic into medaka fertilized eggs using high voltage pulse systems. We assessed the effects of estradiol-17β (E2), bisphenol A (BPA) or BPA relative compounds such as bisphenol S (BPS) for medaka eggs by the incorporation technic and observed by microscope. It was observed that embryonic malformation and abnormal formation of bone, hart, blood vessel and hemocyte chemicals dose-dependently. In addition, we carried out DNA microarray analysis of medaka eggs treated with E2, BPA, BPA relative compounds, and detected variation of each chemicals specific genes expressions. It is suggested that these genes relation to morphological abnormality induced by each chemicals treatment.
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