Genomic and physiological characterization of dehalorespiring bacteria in three phyla

• Project/Area Number: 24710077
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Environmental technology/Environmental materials
• Research Institution: Nagoya Institute of Technology (2013); Toyohashi University of Technology (2012)
• Principal Investigator: YOSHIDA Naoko 名古屋工業大学, 工学(系)研究科(研究院), 助教 (10432220)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 脱ハロゲン化呼吸細菌 / バイオレメディエーション / ジオバクター / デハロバクター / デハロコッコイデス / 還元的脱塩素化 / Geobacter / Dehalobacter / 脱塩素化呼吸 / 還元的脱ハロゲン化

Research Abstract

Dehalorespiring bacteria grow by respiration using organohalides as electron acceptors and reductively dehalogenate organohalides to less halogenated compounds. I have also enriched and isolated phylogenetically and functionally different dehalorespiring bacteria of the three phyla from various environmental samples. Among them, Geobacter sp. AY showed unprecedented high dechlorination activity that detoxify 1,2-dichloroethane (1,2-DCA) of 1,000 ppm to ethene within a week. Geobacter sp. AY had following advantages to be applied in bioremediation; the high activity for high concentration of 1,2-DCA, culturability using only acetate as sole electron donor and carbon source, and the high activity in the culture grown without any organohalides. The genome analysis revealed the constitutive expression was probably resulted from a plasmid lacking of cAMP kinase transcriptional regulator transferred from obligate dehalorespiring Dehalobacter.

Research Products

• [Journal Article] Aquatic plant surface 1 as a niche for methanotrophs (2014)
  • Author(s): N Yoshida, H Iguchi, H Yurimoto, A Murakami, Y Sakai
  • Journal Title: Frontiers in microbiology Volume: 5
• [Journal Article] Anaerobic 4-chlorophenol mineralization in an enriched culture under iron-reducing conditions (2014)
  • Author(s): Li, D Suzuki, C Zhang, N Yoshida, S Yang, A Katayama
  • Journal Title: Journal of Bioscience and Bioengineering Volume: (accepted)
  • NAID: 110009894837
• [Journal Article] Aquatic plant surface as a niche for methanotrophs. (2014)
  • Author(s): Yoshida N, Iguchi H, Yurimoto H, Murakami A, Sakai Y
  • Journal Title: Frontiers in Microbiology / Terrestrial Microbiology Volume: 5 Pages: 1-8
  • DOI: 10.3389/fmicb.2014.00030
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Anaerobic 4-chlorophenol mineralization in an enriched culture under iron-reducing conditions (2014)
  • Author(s): Zhiling Li, Daisuke Suzuki, Chunfang Zhang, Suyin Yang, Naoko Yoshida, and Arata Katayama
  • Journal Title: Journal of Bioscience and Bioengineering Volume: (in press) Issue: 5 Pages: 529-532
  • DOI: 10.1016/j.jbiosc.2014.04.007
  • NAID: 110009894837
  • Peer Reviewed
• [Journal Article] Involvement of Dehalobacter strains in the anaerobic dechlorination of 2,4,6-trichlorophenol (2013)
  • Author(s): Z Li, D Suzuki, C Zhang, N Yoshida, S Yang, A Katayama
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 116 Pages: 602-609
  • NAID: 110009685212
• [Journal Article] A humin-dependent Dehalobacter species is involved in reductive debromination of Tetrabromobisphenol A (2013)
  • Author(s): C Zhang, Z Li, D Suzuki, L Ye, N Yoshida, A Katayama
  • Journal Title: Chemosphere Volume: 92 Pages: 1343-8
• [Journal Article] A humin-dependent Dehalobacter species is involved in reductive debromination of tetrabromobisphenol A (2013)
  • Author(s): Chunfang Zhang, Zhiling Li, Daisuke Suzuki, Lizhen Ye, Naoko Yoshidaaoko, and Arata Katayama
  • Journal Title: Chemosphere Volume: 92 Issue: 10 Pages: 1343-1348
  • DOI: 10.1016/j.chemosphere.2013.05.051
  • Peer Reviewed
• [Journal Article] Evaluation of biodegradable plastics as solid hydrogen donors for the reductive dechlorination of fthalide by Dehalobacter species (2012)
  • Author(s): N Yoshida, L Ye, F Liu, L Zhiling, A Katayama
  • Journal Title: Bioresource Technology Volume: 130 Pages: 478-485
• [Journal Article] Simulation of the Reductive Dechlorination Processes in a Lab-Scale Anaerobic Biobarrier with the Enriched TCP Dechlorination Consortium (2012)
  • Author(s): Zhiling Li, Yasushi Inoue, Takuya Mizoguchi, Yohei Simizu, Naoko Y oshida andArata Katayama
  • Journal Title: Transactions of Tianjin University Volume: 18 Issue: 6 Pages: 441-449
  • DOI: 10.1007/s12209-012-1768-8
  • Peer Reviewed
• [Presentation] Physiological and genomic differences of dehalorespiring bacteria in three different phyla (2013)
  • Author(s): Naoko Yoshida
  • Organizer: The 5th Taiwan-Korea-Japan International Symposium on Microbial Ecology
  • Place of Presentation: National Central University, Taiwan
  • Year and Date: 2013-11-01
  • Invited
• [Presentation] Enrichment of new candidate PCB dechlorinator, Dehalobacter sp. FTH1 and the physiological and genomic characterization (2013)
  • Author(s): Naoko Yoshida
  • Organizer: Jade Mountain Forum on Sustainable Environment
  • Place of Presentation: National Cheng Kung University , Taiwan
  • Year and Date: 2013-04-23
  • Invited
• [Presentation] 細菌によるハロゲン化メチルの代謝 (2012)
  • Author(s): 吉田奈央子,井町寛之,片山新太,由里本博也,阪井康能
  • Organizer: 第216回生存圏シンポジウム植物と微生物大気中のC1化合物を介した気候変動
  • Year and Date: 2012-12-04
  • Invited
• [Presentation] 多様な芳香族塩素化合物を脱塩素化するDehalobacter sp. FTH1株のドラフトゲノム解析に基づく脱ハロゲン化酵素遺伝子解析 (2012)
  • Author(s): 吉田奈央子、Hauke Smidt、片山新太
  • Organizer: 環境バイオテクノロジー学会2013年度大会
  • Year and Date: 2012-06-25
• [Presentation] 多様な芳香族塩素化合物を脱塩素化するDehalobacter sp. FTH1株の ドラフトゲノム解析に基づく脱ハロゲン化酵素遺伝子解析
  • Author(s): 吉田奈央子、Hauke Smidt、片山新太
  • Organizer: 環境バイオテクノロジー学会 2013年度大会
  • Place of Presentation: 京都大学 おうばくプラザ
• [Presentation] Enrichment of new candidate PCB dechlorinator, Dehalobacter sp. FTH1 and the physiological and genomic characterization.
  • Author(s): Naoko Yoshida
  • Organizer: Jade Mountain Forum on Sustainable Environment
  • Place of Presentation: National Cheng Kung University , Taiwan
  • Invited
• [Presentation] 細菌によるハロゲン化メチルの代謝
  • Author(s): 吉田 奈央子, 井町 寛之, 片山 新太, 由里本 博也, 阪井 康能
  • Organizer: 第216回 生存圏シンポジウム 植物と微生物 大気中のＣ1化合物を介した気候変
  • Place of Presentation: 京都大学生存圏研究所
  • Invited