Construction of rigid polymer conjugated oligonucleotide

• Project/Area Number: 24710125
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: Suzuka University of Medical Science
• Principal Investigator: TASHIRO Ryu 鈴鹿医療科学大学, 薬学部, 助教 (00402785)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: ＤＮＡデバイス / ナノテクノロジー / DNA / 核酸化学 / 分子デバイス

Research Abstract

DNA is a powerful tool for creating nanosized architectures and devices owing to its synthetic accessibility and ability to self-assemble. We designed and synthesized a novel H-shaped DNA oligonucleotide dimer cross-linked with a rigid linker. The formation of these motifs was observed via native polyacryla-mide gel analysis and a fluorescence resonance energy transfer experiment using labeled DNA.

Research Products

• [Journal Article] Controlling Electron Rebound Within Four-Base Pi-Stacks in Z-DNA by Changing Sugar Moiety from Deoxy- to Ribonucleotide (2014)
  • Author(s): Sannohe, Y.; Kizaki, S.; Kanesato, S.;Fujiwara, A.;Morinaga, H.; Tashiro, R.;Sugiyama, H.
  • Journal Title: Chem. Eur. J. Volume: 20 Issue: 5 Pages: 1223-1225
  • DOI: 10.1002/chem.201303930
  • Peer Reviewed
• [Journal Article] Photoreactivities of 5-Bromouracil-containing RNAs (2013)
  • Author(s): Morinaga H, Kizaki S, Takenaka T, Kanesato S, Sannohe Y, Tashiro R, Sugiyama H.
  • Journal Title: Bioorg Med Chem Volume: 21 Issue: 2 Pages: 466-469
  • DOI: 10.1016/j.bmc.2012.11.010
  • Peer Reviewed
• [Presentation] 剛直なリンカーで連結されたDNAの合成 (2014)
  • Author(s): 田代 竜、岩本 真拡、杉山 弘
  • Organizer: 日本化学会 第９４回春期年会
  • Place of Presentation: 名古屋大学 東山キャンパス
• [Presentation] 剛直なリンカーで連結されたDNAの合成
  • Author(s): 田代竜・岩本真拡・杉山弘
  • Organizer: 第94回日本化学会春年会口頭発表