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Fabrication of Solid-Supported Biological Membrane Models Using Cell-Free Protein Synthesis

Research Project

Project/Area Number 24710127
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Nanomaterials/Nanobioscience
Research InstitutionNational Institute for Materials Science

Principal Investigator

KATAOKA Chiho  独立行政法人物質・材料研究機構, 国際ナノアーキテクトニクス研究拠点, 研究員 (70443009)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Keywords生体膜 / ベシクル / 基盤支持脂質膜 / 支持脂質膜 / 蛍光顕微鏡 / 基板支持脂質膜 / 膜蛋白質 / 界面活性剤 / 無細胞合成 / 膜タンパク質
Outline of Final Research Achievements

In this project, the reconstitution of transmembrane proteins in supported lipid bilayers using cell-free protein synthesis was investigated. Supported bilayers were formed by vesicle fusion, and then protein expression was conducted over these bilayer systems. However, protein folding in supported bilayers was not observed under any experimental conditions tested. The project also aimed at the investigation of properties of supported lipid bilayers because they affect the properties and functions of transmembrane proteins. It was found that the packing density of supported bilayers can change with solution pH. In addition, the defect formation mechanisms in supported bilayers were clarified.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (5 results)

All 2015 2014

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 2 results) Presentation (3 results)

  • [Journal Article] Induced Rupture of Vesicles Adsorbed on Glass by Pore Formation at the Surface-Bilayer Interface2015

    • Author(s)
      Chiho Kataoka-Hamai, Tomohiko Yamazaki
    • Journal Title

      Langmuir

      Volume: 31 Issue: 4 Pages: 1312-1319

    • DOI

      10.1021/la5042822

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Packing Density Changes of Supported Lipid Bilayers Observed by Fluorescence Microscopy and Quartz Crystal Microbalance-Dissipation2014

    • Author(s)
      Chiho Kataoka-Hamai, Mahoko Higuchi
    • Journal Title

      Journal of Physical Chemistry B

      Volume: 118 Issue: 37 Pages: 10934-10944

    • DOI

      10.1021/jp503905r

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] ガラスに吸着したベシクルの開裂:吸着領域での孔形成が誘発する機構2015

    • Author(s)
      片岡 知歩
    • Organizer
      日本化学会第95春季年会
    • Place of Presentation
      日本大学(千葉県船橋市)
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 吸着ベシクルの開裂機構:吸着領域での欠陥孔形成が関わる機構2015

    • Author(s)
      片岡 知歩、山崎 智彦
    • Organizer
      第62回応用物理学会春季学術講演会
    • Place of Presentation
      東海大学(神奈川県平塚市)
    • Year and Date
      2015-03-11 – 2015-03-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] 基板支持脂質膜の分子密度変化2014

    • Author(s)
      片岡 知歩
    • Organizer
      第65回コロイドおよび界面化学討論会
    • Place of Presentation
      東京理科大学(東京都新宿区)
    • Year and Date
      2014-09-03 – 2014-09-05
    • Related Report
      2014 Annual Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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