Fabrication of Solid-Supported Biological Membrane Models Using Cell-Free Protein Synthesis
| Project/Area Number |
24710127
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Nanomaterials/Nanobioscience
|
|---|
| Research Institution | National Institute for Materials Science |
|---|
Principal Investigator |
KATAOKA Chiho 独立行政法人物質・材料研究機構, 国際ナノアーキテクトニクス研究拠点, 研究員 (70443009)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
|
|---|
| Keywords | 生体膜 / ベシクル / 基盤支持脂質膜 / 支持脂質膜 / 蛍光顕微鏡 / 基板支持脂質膜 / 膜蛋白質 / 界面活性剤 / 無細胞合成 / 膜タンパク質 |
|---|
| Outline of Final Research Achievements |
In this project, the reconstitution of transmembrane proteins in supported lipid bilayers using cell-free protein synthesis was investigated. Supported bilayers were formed by vesicle fusion, and then protein expression was conducted over these bilayer systems. However, protein folding in supported bilayers was not observed under any experimental conditions tested. The project also aimed at the investigation of properties of supported lipid bilayers because they affect the properties and functions of transmembrane proteins. It was found that the packing density of supported bilayers can change with solution pH. In addition, the defect formation mechanisms in supported bilayers were clarified.
|
Report
(4 results)
Research Products
(5 results)
