Development of glycan immobilization technique for virus detection and its device applications

• Project/Area Number: 24710136
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: Waseda University
• Principal Investigator: HIDESHIMA SHO 早稲田大学, ナノ理工学研究機構, 講師 (10580433)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ナノバイオ / 糖鎖 / バイオセンシング / 電界効果トランジスタバイオセンサ / インフルエンザウイルス / 電界効果トランジスタ / ウイルス

Research Abstract

I have developed a glycan immobilization technique for biosensing devices based on filed effect transistors towards influenza virus detection. Highly dense and oriented glycan immobilization has been achieved by introducing an aminooxy-terminated self-assembled monolayer on the sensing surface. The glycan-immobilized FET could detect influenza virus hemagglutinin at attomolar-level sensitivity. The results suggest that the glycan-immobilized FETs developed in the project have potential to detect influenza virus with the high sensitivity.

Research Products

• [Journal Article] Monitoring Amyloid Sup35NM Growth with Label-Free Electrical Detection Using Field Effect Transistor Biosensor (2014)
  • Author(s): S. Hideshima, S. Wustoni, S. Kuroiwa, T. Nakanishi, A. K-Takeike, T. Osaka
  • Journal Title: ChemElectroChem Volume: 1 Issue: 1 Pages: 51-54
  • DOI: 10.1002/celc.201300151
  • Peer Reviewed
• [Journal Article] A label-free electrical assay of fibrous amyloid β based on semiconductor biosensing (2014)
  • Author(s): S. Hideshima, M. Kobayashi, T. Wada, S. Kuroiwa, T. Nakanishi, N. Sawamura, T. Asahi, T. Osaka
  • Journal Title: Chemical Communications Volume: 50 Issue: 26 Pages: 3476-3479
  • DOI: 10.1039/c3cc49460h
  • Peer Reviewed
• [Journal Article] Field effect transistor biosensor using antigen binding fragment for detecting tumor marker in human serum (2014)
  • Author(s): S. Cheng, K. Hotani, S. Hideshima, S. Kuroiwa, T. Nakanishi, M. Hashimoto, Y. Mori, T. Osaka
  • Journal Title: Materials Volume: 7 Issue: 4 Pages: 2490-2500
  • DOI: 10.3390/ma7042490
  • Peer Reviewed
• [Journal Article] Attomolar-level Detection of Influenza A Virus Hemagglutinin Human H1 and Avian H5 Using Glycan-blotted Field Effect Transistor Biosensor (2013)
  • Author(s): Sho Hideshima, Hiroshi Hinou, Daisuke Ebihara, Ryosuke Sato, Shigeki Kuroiwa, Takuya Nakanishi, Shin-Ichiro Nishimura, Tetsuya Osaka
  • Journal Title: Analytical Chemistry Volume: 85 Issue: 12 Pages: 5641-5644
  • DOI: 10.1021/ac401085c
  • Peer Reviewed
• [Journal Article] Effect of the size of Receptor in Allergy Detection Using Field Effect Transistor Biosensor (2013)
  • Author(s): S. Hideshima, S. Kuroiwa, M. Kimura, S. Cheng, T. Osaka
  • Journal Title: Electrochimica Acta Volume: 110 Pages: 146-151
  • DOI: 10.1016/j.electacta.2013.07.113
  • Peer Reviewed
• [Presentation] 糖鎖受容体を用いた電界効果トランジスタによるインフルエンザウイルスヘマグルチニンの検出 (2013)
  • Author(s): 海老原大介, 秀島翔, 比能洋, 黒岩繁樹, 中西卓也, 西村紳一郎, 逢坂哲彌
  • Organizer: 第3回CSJ化学フェスタ2013
  • Place of Presentation: 東京
  • Year and Date: 2013-10-22
• [Presentation] 糖鎖を用いた半導体バイオセンシング―インフルエンザウイルス検出に向けた研究開発 (2013)
  • Author(s): 秀島翔
  • Organizer: 第7回GFRG研究会シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2013-08-28
• [Presentation] Carbohydrate Immobilization on the Surface of Field Effect Transistor Biosensor for Detection of Virus-related Protein (2012)
  • Author(s): S. Hideshima, H. Hinou, D. Ebihara, R. Sato, S. Kuroiwa, S. Nishimura, T. Osaka
  • Organizer: Pacific Rim Meeting on Electrochemical and Solid-State Science (PRiME) 2012
  • Place of Presentation: Honolulu, Hawaii, USA
  • Year and Date: 2012-10-09
• [Presentation] Response of Field Effect Transistor Biosensor to Amyloid Beta (1-42) Depending on Its Growth
  • Author(s): S. Hideshima, M. Kobayashi, S. Kuroiwa, T. Nakanishi, N. Sawamura, A. Toru, T. Osaka
  • Organizer: 64th International Society of Electrochemistry
  • Place of Presentation: Santiago de Queretaro, Mexico
• [Presentation] Effect of Amyloid Conformation On the Response of Field Effect Transistor Biosensor to Sup35NM Protein
  • Author(s): S. Hideshima, S. Wustoni, S. Kuroiwa, T. Nakanishi, A. Koike, T. Osaka
  • Organizer: 224th Meeting of the Electrochemical Society
  • Place of Presentation: San Francisco, USA
• [Presentation] 糖鎖受容体を用いた電界効果トランジスタによるインフルエンザウイルスヘマグルチニンの検出
  • Author(s): 海老原大介、秀島翔、比能洋、黒岩繁樹、中西卓也、西村紳一郎、逢坂哲彌
  • Organizer: 第3回CSJ化学フェスタ 2013
  • Place of Presentation: 東京
• [Presentation] Carbohydrate Immobilization on the Surface of Field Effect Transistor Biosensor for Detection of Virus-related Protein
  • Author(s): S. Hideshima, H. Hinou, D. Ebihara, R. Sato, S. Kuroiwa, S. Nishimura, T. Osaka
  • Organizer: PRiME 2012 (222nd Meeting of The Electrochemical Society, 2012 Fall Meeting of The Electrochemical Society of Japan)
  • Place of Presentation: Honolulu, Hawaii, USA
• [Remarks] 本研究課題の成果に関する報道が、日本経済新聞(2013年6月20日、2013年11月23日、及び2014年3月25日)に掲載・紹介された。また、同研究内容がブラジル全国ネットのテレビ局RECORD TVにて放映・紹介された(2013年7月13日)。
• [Remarks]
  • URL: http://www.ec.appchem.waseda.ac.jp/INDEXJ.HTM
• [Patent(Industrial Property Rights)] 糖化合物固定化半導体センシングデバイス及び生物学的物質の検出方法 (2012)
  • Inventor(s): 逢坂哲彌, 秀島翔, 黒岩繁樹, 西村 紳一郎, 比能洋
  • Industrial Property Rights Holder: 学校法人 早稲田大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2012-191701
  • Filing Date: 2012-08-31
• [Patent(Industrial Property Rights)] 糖化合物固定化半導体センシングデバイス及び生物学的物質の検出方法 (2012)
  • Inventor(s): 逢坂哲彌, 秀島翔, 黒岩繁樹, 西村紳一郎, 比能洋
  • Industrial Property Rights Holder: 学校法人早稲田大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2012-191701
  • Filing Date: 2012-08-31