Development of peptide array for high sensitive detection of phosphorylation signal using MALDI-MS
| Project/Area Number |
24710137
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | Kitakyushu National College of Technology |
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Principal Investigator |
SONODA Tatsuhiko 北九州工業高等専門学校, 物質化学工学科, 准教授 (30403992)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ペプチドチップ / リン酸化シグナル / 質量分析 / 酸化チタン / セルフクリーニング / ペプチドアレイ / プロテインキナーゼ / MALDI-TOF-MS |
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| Research Abstract |
A peptide array using a titanium oxide plate has been investigated for detection of protein kinases activities. Because the one has self-cleaning ability to remove non-specific adsorption on the surface of itself, and plays a role of inorganic matrix at MALDI mass spectrometry, it will be expected to be able to realize more accurate analysis of protein kinases activities with high sensitivity. First, the titanium oxide plate was prepared by sol-gel method. It showed photo-induced hydrophilicity at UV irradiation and much of non-specific adsorption on its surface was removed only by washing with water. Next, substrate peptides were immobilized on the surface using 3-aminopropyl triethoxysilane and photo-cleaved linker by laser equipped with a MALDI mass spectrometer (MS). Finally, the peptide array were analyzed by MS. From above results, we would establish the basis technique to develop the peptide array using the titanium oxide plate for high sensitive detection at MALDI-MS.
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Report
(3 results)
Research Products
(8 results)
