| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Research Abstract |
The objective of this study was to reconstruct multilayered structure of micro organization using dielectrophoresis (DEP). DEP is a phenomenon in which a force is generated on a cell or particle when it is subjected to a non-uniform electric field. In this study, alginate-collagen micro-beads were developed for dielectrophoresis experiments and cell adhesive scaffold. Dermal and epi-dermal skin cells were assembled by DEP force to cover the beads and to construct multilayered structure. After the cell assembly, the cell-accumulated beads were cultured in vitro to reconstruct maultilayered skin tissue. Finally, the cell viability and proliferation of the cultured constructs were maintained during 7 days culture.
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