Role of acetylation of transcription factor CRP in network of metabolic regulation

• Project/Area Number: 24710214
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Genome biology
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: SHIMADA Tomohiro 東京工業大学, 資源化学研究所, 助教 (10535230)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: CRP / アセチル化 / RMF / 100Sリボソーム / Genomic SELEX / RNAポリメラーゼ / ゲノム転写制御 / 大腸菌 / 代謝制御 / 転写因子CRP

Outline of Final Research Achievements

It was observed that the variation of acetylation level of CRP (cAMP receptor protein) along growth phase and examined the effect of acetylated CRP for promoter activity of under regulated genes in Escherichia coli. We succeeded the identification of the genome distribution of CRP and RNA polymerase. It is useful to understand the molecular mechanism of transcriptional regulation of E.coli genome. It is also revealed that CRP enhanced formation of 100S ribosome by transcriptional activation of rmf (ribosome modulation factor).

Research Products

• [Journal Article] Roles of cell division control factor SdiA: recognition of quorum sensing signals and modulation of transcription regulation targets (2014)
  • Author(s): Tomohiro Shimada1,2,3, Kaori Shimada, Makoto Matsui, Yuichi Kitai, Jun Igarashi, Hiroaki Suga, Akira Ishihama
  • Journal Title: Genes to Cells Volume: 19 Issue: 5 Pages: 405-418
  • DOI: 10.1111/gtc.12139
  • Peer Reviewed / Open Access
• [Journal Article] Unprecedented high-resolution view of bacterial operon architecture revealed by RNA sequencing. (2014)
  • Author(s): Conway T, Creecy JP, Maddox SM, Grissom JE, Conkle TL, Shadid TM, Teramoto J, San Miguel P, Shimada T, Ishihama A, Mori H, Wanner BL.
  • Journal Title: MBio Volume: 5 Issue: 4
  • DOI: 10.1128/mbio.01442-14
  • Peer Reviewed / Open Access
• [Journal Article] The whole set of constitutive promoters recognized by RNA polymerase RpoD holoenzyme of Escherichia coli (2014)
  • Author(s): Tomohiro Shimada, Yukiko Yamazaki, Kan Tanaka and Akira Ishihama
  • Journal Title: PLos One Volume: 9 Issue: 3 Pages: e90447-e90447
  • DOI: 10.1371/journal.pone.0090447
  • Peer Reviewed
• [Journal Article] Regulation of the rmf gene encoding ribosome modulation factor in Escherichia coli: Involvement of cAMP receptor protein. (2013)
  • Author(s): Shimada, T., Yhoshida, H. and Ishihama, A.
  • Journal Title: Journal of Bacteriology Volume: 印刷中
  • Peer Reviewed
• [Journal Article] A novel regulator PgrR for switch control of peptideglycan recycling in Escherichia coli. (2012)
  • Author(s): Shimada, T., Yamazaki, K. and Ishihama, A.
  • Journal Title: Genes to Cells Volume: 18 Issue: 2 Pages: 123-134
  • DOI: 10.1111/gtc.12026
  • Peer Reviewed
• [Presentation] 大腸菌におけるメタボリックスイッチ制御機構の解明 (2015)
  • Author(s): 島田友裕、山梨智也、鮒信学、田中寛
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山大学津島キャンパス
  • Year and Date: 2015-03-27
• [Presentation] 大腸菌RNAポリメラーゼRpoDホロ酵素によって認識されるConstitutive promotersの探索と解析 (2014)
  • Author(s): 島田 友裕, 山崎 由紀子, 田中 寛, 石浜 明
  • Organizer: ２０１４年日本ゲノム微生物学会
  • Place of Presentation: 東京農業大学
• [Presentation] 大腸菌炭素源代謝グローバルレギュレーターCRPの新規機能 (2013)
  • Author(s): 島田 友裕
  • Organizer: 国立遺伝学研究所 2013年度研究集会
  • Place of Presentation: 国立遺伝学研究所
• [Presentation] The Whole Set of Constitutive Promoters Recognized by RNA polymerase RpoD Holoenzyme of Escherichia coli (2013)
  • Author(s): Tomohiro Shimada, Yukiko Yamazaki, Kan Tanaka and Akira Ishihama
  • Organizer: 第３６回日本分子生物学会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] 大腸菌ゲノムのサイレンシング機構 (2012)
  • Author(s): 島田友裕、山崎由紀子、石浜 明
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場
• [Presentation] 大腸菌をモデル生物とした細胞の代謝・増殖制御機構の解明 (2012)
  • Author(s): 島田友裕、斎藤菜摘、田中 寛
  • Organizer: 第七回メタボロームシンポジウム
  • Place of Presentation: 慶応義塾大学
• [Remarks] 東京工業大学 資源化学研究所 田中・今村研究室ホームページ
  • URL: http://www.res.titech.ac.jp/~biores/index.html
• [Remarks] 東京工業大学資源化学研究所生物資源部門
  • URL: http://www.res.titech.ac.jp/~biores/index.html
• [Remarks] 東京工業大学資源化学研究所生物資源部門 発表成果
  • URL: http://www.res.titech.ac.jp/~biores/pg192.html