Role of acetylation of transcription factor CRP in network of metabolic regulation
| Project/Area Number |
24710214
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genome biology
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | CRP / アセチル化 / RMF / 100Sリボソーム / Genomic SELEX / RNAポリメラーゼ / ゲノム転写制御 / 大腸菌 / 代謝制御 / 転写因子CRP |
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| Outline of Final Research Achievements |
It was observed that the variation of acetylation level of CRP (cAMP receptor protein) along growth phase and examined the effect of acetylated CRP for promoter activity of under regulated genes in Escherichia coli. We succeeded the identification of the genome distribution of CRP and RNA polymerase. It is useful to understand the molecular mechanism of transcriptional regulation of E.coli genome. It is also revealed that CRP enhanced formation of 100S ribosome by transcriptional activation of rmf (ribosome modulation factor).
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Unprecedented high-resolution view of bacterial operon architecture revealed by RNA sequencing.2014
Author(s)
Conway T, Creecy JP, Maddox SM, Grissom JE, Conkle TL, Shadid TM, Teramoto J, San Miguel P, Shimada T, Ishihama A, Mori H, Wanner BL.
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Journal Title
DOI
Related Report
Peer Reviewed / Open Access
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