| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Outline of Final Research Achievements |
Chromatin insulators partition the genome into functional units to control cell type- and developmental stage-specific gene expression. The CCCTC-binding factor (CTCF) is an insulator-binding protein that functions in transcriptional regulation and higher-order chromatin formation. CTCF is enriched at several tens of thousands of sites on the human genome. However, only a part of the sites can function as insulators in cell type- and developmental stage-specific manner. In this study, to know the role of insulators at the human HOXA gene locus, we generated insulator-targeted cells by genome editing with engineered ZFNs. Our results showed that insulator is responsible for higher-order chromatin formation and proper gene expression. Additionally, we characterized a candidate for a novel transcriptional factor that binds to the functional insulator site in the human HOXA gene locus.
|
