| Project/Area Number |
24710218
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genome biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SATO Kaoru 東京大学, 理学(系)研究科(研究院), 助教 (20548507)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | RNAサイレンシング / piRNA / トランスポゾン / 生殖細胞 / Krimp / ショウジョウバエ / RNA干渉 / 細胞内顆粒 / 国際情報交換 |
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| Research Abstract |
piRNA is a germline-specific class of small non-coding RNA, and functions by specifically interacting with PIWI proteins. In germline cells, piRNA thereby suppresses the expression of transposable elements, so piRNA is thought to protect the germline genome from such harmful element. piRNAs are thought to be produced by two distinct mechanisms, primary pathway and secondary pathway. A Drosophila PIWI protein AGO3 interacts with piRNAs produced only by the secondary pathway. In this study, I found that a Drosophila piRNA factor Krimper (Krimp) specifically interacts with AGO3 and regulates its piRNA production, indicating that Krimp is a factor regulating the secondary pathway. In addition, I found that Krimp regulates piRNAs are not loaded on AGO3 by the primary pathway.
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