| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Research Abstract |
Arabidopsis contain a gene coding for single predicted transmembrane helix protein called OHP1 with a light-harvesting chlorophyll a/b-binding (LHC) motif. Significant amounts of OHP1 protein were present in green tissue but their level increased in response to high-light stress. In this study we found that ohp1 mutants showed pale-green phenotype on agar plates. Leaf chlorophyll fluorescence analysis and thylakoid membrane composition indicated that ohp1 mutants had defects in PSII supercomplexes, but did not affect the accumulation of LHCs. We also generated transgenic plants expressing the tag fusion protein in ohp1 mutant that complement its pale-green phenotype. From the results of several biochemical analyses for revealing the localization of OHP1 on thylakoid membrane, the association of LHC-II with the OHP proteins has been deduced. These results suggest that OHP1 plays a fundamental role in assembly of photosystem II and is working around the LHCII SCPs as oligomers.
|
