| Project/Area Number |
24710221
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical genome science
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| Research Institution | Hiroshima University |
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Principal Investigator |
HOZUMI Shunya 広島大学, 理学(系)研究科(研究院), 助教 (10597222)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 分化転換 / 神経細胞 / 内胚葉 / 中胚葉 / 遺伝子発現 / Notch / エピジェネティクス / 細胞運命 / 中枢神経系 / Notch情報伝達系 / ゼブラフィッシュ / 網膜 / 脊椎 / 中内胚葉 / 遺伝子発現制御 |
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| Outline of Final Research Achievements |
It is well known that three primary germ layer cells, known as ectoderm, mesoderm, and endoderm, are differentiated from pluripotent stem cells in vertebrates through the induction of cell lineage specific gene expression. Although it was thought that the linage-specified differentiated cells are very stable, recent studies revealed that the differentiated cells could be reprogrammed by overexpression of germ layer specific transcription factors, called direct reprogramming, which changes the profiles of gene expression. However, it is still difficult to convert one germ layer cells into another germ layer fate. In this study, I found that inhibition of Notch signaling ectopically induces expression of mesodermal and endodermal genes, such as pancreatic genes, in zebrafish central nervous system. These results will help to promote the direct conversion technology in regenerative medicine.
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