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Study on a novel folate biosynthesis pathway on micoorganism

Research Project

Project/Area Number 24710239
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Living organism molecular science
Research InstitutionHokkaido University

Principal Investigator

SATOH Yasuharu  北海道大学, 工学(系)研究科(研究院), 助教 (30360928)

Co-Investigator(Renkei-kenkyūsha) DAIRI Tohru  北海道大学, 大学院工学研究院, 教授 (70264679)
KURATSU Masahiro  協和発酵バイオ(株), 研究員
Research Collaborator OBAYASHI Daiki  北海道大学, 工学部, 学部4年
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywords葉酸 / アミノ安息香酸 / ニトロソモナス / クラミジア / アンモニア酸化菌 / 偏性細胞内寄生菌 / para-aminobenzoic acid / folate / Nitrosomonas / Chlamydia / ammonia-oxidizing / Thaumarchaeota / パラアミノ安息香酸 / Xanthomonas / ゲノムマイニング
Research Abstract

Folate is an essential cofactor in all living cells. para-Aminobenzoate (pABA), a building block of folate, is usually derived from chorismate in the shikimate pathway by reactions of PabABC. We previously suggested that an alternative pathway for pABA biosynthesis would operate in some microorganisms such as Lactobacillus fermentum and Nitrosomonas europaea since these bacteria showed a prototrophic phenotype to pABA despite the fact that there are no orthologs of pabABC in their genome databases. In this study, a gene of unknown function, NE1434, was obtained from N. europaea by shotgun cloning using a pABA-auxotrophic Escherichia coli mutant (dpabABC) as a host. A tracer experiment using [U-13C6]glucose suggested that pABA was de novo synthesized in the transformant. An E. coli dpabABCdaroB mutant carrying the NE1434 gene exhibited a prototrophic phenotype to pABA, suggesting that compounds in the shikimate pathway including chorismate were not utilized as substrates by NE1434.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (5 results)

All 2014 2013

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (4 results)

  • [Journal Article] New gene responsible for para-aminobenzoate biosynthesis2014

    • Author(s)
      Satoh, Y., Kuratsu, M., Kobayashi, D., Dairi, T.
    • Journal Title

      J. Biosci. Bioeng

      Volume: 117(2) Issue: 2 Pages: 178-183

    • DOI

      10.1016/j.jbiosc.2013.07.013

    • NAID

      110010039563

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Presentation] Novel folate biosynthesis pathway2014

    • Author(s)
      SATOH, Y., DAIRI, T.
    • Organizer
      US-Japan seminar on the biosynthesis of natural products for young researcher
    • Place of Presentation
      Tokyo Institute of Technology
    • Year and Date
      2014-03-02
    • Related Report
      2013 Final Research Report
  • [Presentation] Novel folate biosynthetic pathway2014

    • Author(s)
      Yasuharu SATOH, Tohru DAIRI
    • Organizer
      US-Japan Seminar on the Biosynthesis of Natural Products for Young Researchers
    • Place of Presentation
      Tokyo Institute of Technology(東京都)
    • Related Report
      2013 Annual Research Report
  • [Presentation] バクテリアにおける新規葉酸生合成経路の解明2013

    • Author(s)
      佐藤康治, 小林大毅, 大利徹
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      東北大学
    • Year and Date
      2013-03-25
    • Related Report
      2013 Final Research Report
  • [Presentation] バクテリアにおける新規葉酸生合成経路の解明2013

    • Author(s)
      佐藤康治,小林大毅,大利徹
    • Organizer
      日本農芸化学2013年度大会
    • Place of Presentation
      東北大学 宮城県仙台市
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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