Development of a novel ligand screening system for G protein-coupled receptors and its application
| Project/Area Number |
24710247
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Living organism molecular science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
IIZUKA Ryo 東京大学, 薬学研究科(研究院), 助教 (90541954)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | Gタンパク質共役受容体 / 進化分子工学 / 出芽酵母 / マイクロ流体デバイス / エマルジョン / 分子進化 / リガンド探索 |
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| Outline of Final Research Achievements |
G protein-coupled receptors (GPCRs) are particularly important drug targets. Thus, intense efforts have been devoted to screening novel ligands for GPCRs, which can lead to potential drug candidates. However, for many GPCRs, such efforts have failed to yield desired results. To overcome the problem, I envisioned a directed evolution system to generate agonistic peptides for GPCRs using water-in-oil emulsions. In this study, I established basic techniques for efficient implementation of the system.
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Report
(4 results)
Research Products
(23 results)
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[Presentation] Peptide-based ligand screening system for G protein-coupled receptors using water-in-oil droplets.2015
Author(s)
Takashi Sakurai, Ryo Iizuka, Rui Sekine, Yasuyuki Nakamura, Dong Hyun Yoon, Tetsushi Sekiguchi, Jun Ishii, Akihiko Kondo, Shuichi Shoji, and Takashi Funatsu
Organizer
International Workshop on Extended-nano Fluidics
Place of Presentation
Koshiba Hall, The University of Tokyo (Tokyo)
Year and Date
2015-03-26 – 2015-03-27
Related Report
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[Presentation] PEPTIDE-BASED LIGAND SCREENING SYSTEM FOR G PROTEIN-COUPLED RECEPTORS USING WATER-IN-OIL MICRODROPLETS.2014
Author(s)
T. Sakurai, R. Iizuka, R. Sekine, Y. Nakamura, D. H. Yoon, T. Sekiguchi, J. Ishii, A. Kondo, S. Shoji, and T. Funatsu
Organizer
The 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014)
Place of Presentation
Henry B. Gonzalez Convention Center (San Antonio, TX, USA)
Year and Date
2014-10-26 – 2014-10-30
Related Report
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[Presentation] Yeast-based fluorescence assay system for detecting G protein-coupled receptor activation in water-in-oil droplets2013
Author(s)
Takashi Sakurai, Ryo Iizuka, Yasuyuki Tanigaki, Rui Sekine, Dong Hyun Yoon, Tetsushi Sekiguchi, Jun Ishii, Akihiko Kondo, Naoto Nemoto, Shuichi Shoji, Takashi Funatsu
Organizer
8th Asian Biophysics Association Symposium
Place of Presentation
Ramada Plaza Jeju, Korea
Related Report
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