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Biosynthetic study of manumycin antibiotics for creating novel analogs

Research Project

Project/Area Number 24710253
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Living organism molecular science
Research InstitutionTokushima Bunri University

Principal Investigator

ITO Takuya  徳島文理大学, 薬学部, 講師 (90517484)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords放線菌 / mutasynthesis / 生合成 / 遺伝子工学
Research Abstract

Sequence analysis of genome from asukamycin producing strain revealed a gene cluster involve in the biosynthesis of asukamycin. Genetic disruption of cyclohexane synthase (asuB3) and 5-aminolevulinate synthase (asuD2), individually abrogated asukamycin production, this confirmed the involvement of the asu gene cluster in asukamycin biosynthesis. Mutasynthesis approach using these mutants was developed for the generation of novel asukamycin analogs.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (36 results)

All 2014 2013 2012 Other

All Journal Article (14 results) (of which Peer Reviewed: 7 results,  Open Access: 1 results) Presentation (19 results) (of which Invited: 1 results) Remarks (3 results)

  • [Journal Article] Two novel aromatic glucosides, marylaurencinosides D and E, from the fresh flowers of Cymbidium Great Flower 'Marylaurencin2014

    • Author(s)
      Yoshikawa, K., Okahuji, M., Iseki, K., Ito, T., Asakawa, Y., Kawano, S., Hashimoto, T.
    • Journal Title

      J. Nat. Med.

      Volume: 68 Issue: 2 Pages: 455-458

    • DOI

      10.1007/s11418-013-0814-8

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Pungent and bitter, cytotoxic and antiviral terpenoids from some bryophytes and inedible fungi.2014

    • Author(s)
      Asakawa, Y., Nagashima, F., Hashimoto, T., Toyota, M., Ludwiczuk, A., Komala, I., Ito, T., Yagi, Y.
    • Journal Title

      Nat. Prod. Commun.

      Volume: 9 Pages: 409-417

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biosynthetic Study of Actinomycetes-Metabolites for Creating Novel Analogs2013

    • Author(s)
      Ito, T.
    • Journal Title

      YAKUGAKU ZASSHI

      Volume: 133 Issue: 9 Pages: 1007-1015

    • DOI

      10.1248/yakushi.13-00175

    • NAID

      130003361989

    • ISSN
      0031-6903, 1347-5231
    • Year and Date
      2013-09-01
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biosynthetic study of actinomycetes- metabolites for creating novel analogs2013

    • Author(s)
      Ito, T.
    • Journal Title

      Yakugaku Zasshi

      Volume: 133 Pages: 1007-1015

    • NAID

      130003361989

    • Related Report
      2013 Final Research Report
  • [Journal Article] Aromatic Constituents of Cymbidium Great Flower Marie Laurencin and their Antioxidative Activity2013

    • Author(s)
      Yoshikawa, K., Otsu, M., Ito, T., Asakawa, Y., Kawano, S., Hashimoto, T.
    • Journal Title

      J. Nat. Med

      Volume: 67 Pages: 217-221

    • NAID

      10031131622

    • Related Report
      2013 Final Research Report
  • [Journal Article] Aromatic Constituents of Cymbidium Great Flower Marie Laurencin and their Antioxidative Activity2013

    • Author(s)
      Yoshikawa, K., Otsu, M., Ito, T., Asakawa, Y., Kawano, S., Hashimoto, T.
    • Journal Title

      J. Nat. Med.

      Volume: 67 Issue: 1 Pages: 217-221

    • DOI

      10.1007/s11418-012-0653-z

    • NAID

      10031131622

    • Related Report
      2013 Annual Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Transcriptional Regulation and Increased Production of Asukamycin in Engineered Streptomyces nodosus subsp. asukaensis Strains2012

    • Author(s)
      Xie, P., Sheng, Y., Ito, T., Mahmud, T.
    • Journal Title

      Appl. Microbiol. Biotech

      Volume: 96 Pages: 451-460

    • Related Report
      2013 Final Research Report
  • [Journal Article] -hydroxyl fatty acids from a metagenomic library of a marine sponge2012

    • Author(s)
      He, R., Wakimoto, T., Egami, Y., Kenmoku, H., Ito, T., Asakawa, Y., Abe, I.
    • Journal Title

      Bioorgan Med. Chem Lett

      Volume: 22 Pages: 7322-7325

    • Related Report
      2013 Final Research Report
  • [Journal Article] Biosynthetic pathway for highly structural diversity of a common dilactone core in antimycin production2012

    • Author(s)
      Yan, Y., Zhang, L., Ito, T., Qu, X., Asakawa, Y., Awakawa, T., Abe, I., Liu, Wen.
    • Journal Title

      Org. Lett

      Volume: 14 Pages: 4142-4145

    • Related Report
      2013 Final Research Report
  • [Journal Article] Porphyrins from a metagenomic library of the marine sponge2012

    • Author(s)
      He, R., Wakimoto, T., Takeshige, Y., Egami, Y., Kenmoku, H., Ito, T., Wang, B., Asakawa, Y., Abe, I.
    • Journal Title

      Molecular BioSystems

      Volume: 8 Pages: 2334-2338

    • Related Report
      2013 Final Research Report
  • [Journal Article] Phenanthrene Derivatives from Cymbidium Great Flower Marie Laurencin and Their Biological Activities2012

    • Author(s)
      Yoshikawa, K., Ito, T., Iseki, K., Baba,C., Imagawa, H., Yagi, Y., Morita, H., Asakawa,Y., Kawano, S., Hashimoto, T.
    • Journal Title

      J. Nat. Prod

      Volume: 75 Pages: 605-609

    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Journal Article] Heterologously expressed β-hydroxyl fatty acids from a metagenomic library of a marine sponge2012

    • Author(s)
      R. He, T. Wakimoto, Y. Egami, H. Kenmoku, T. Ito, Y. Asakawa, I. Abe
    • Journal Title

      Bioorg. Med. Chem. Lett.

      Volume: 22 Issue: 24 Pages: 7322-7325

    • DOI

      10.1016/j.bmcl.2012.10.082

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Biosynthetic pathway for highly structural diversity of a common dilactone core in antimycin production2012

    • Author(s)
      Yan, Y.
    • Journal Title

      Organic Letters

      Volume: 14 Issue: 16 Pages: 4142-4145

    • DOI

      10.1021/ol301785x

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Porphyrins from a metagenomic library of the marine sponge Discodermia calyx2012

    • Author(s)
      He, R.
    • Journal Title

      Mol. BioSyst.

      Volume: 6 Issue: 9 Pages: 2334-2338

    • DOI

      10.1039/c2mb25169h

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] メディオマイシン生合成遺伝子の同定2014

    • Author(s)
      張驪駻, 伊藤卓也, 脇本敏幸, 淡川孝義, 浅川義範, 阿部郁朗
    • Organizer
      日本薬学会第134年会
    • Place of Presentation
      熊本
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 本邦産,マレーシア産およびアルゼンチン産苔類のテルペノイド2014

    • Author(s)
      長島史裕, 井上進之介, 上田淳也, 新田佳代, 仲松早希, 八木康行, 伊藤卓也, 浅川義範
    • Organizer
      日本薬学会第134年会
    • Place of Presentation
      熊本
    • Related Report
      2013 Final Research Report
  • [Presentation] Oridamycin生合成遺伝子のクローニング2014

    • Author(s)
      川崎崇, 渡邊あゆみ, 伊藤卓也, 今村信孝
    • Organizer
      日本薬学会第134年会
    • Place of Presentation
      熊本
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 本邦産,マレーシア産およびアルゼンチン産苔類のテルペノイド2014

    • Author(s)
      長島史裕、井上進之介、上田淳也、新田佳代、仲松早希、八木康行、伊藤卓也、浅川義範
    • Organizer
      日本薬学会第134年会
    • Place of Presentation
      熊本
    • Related Report
      2013 Annual Research Report
  • [Presentation] 放線菌由来新規化合物の探索2014

    • Author(s)
      山田陽香、佐藤道大、伊藤卓也、高橋公咲、野口博司、渡辺賢二
    • Organizer
      第60 回日本生薬学会
    • Place of Presentation
      北海道
    • Related Report
      2013 Annual Research Report
  • [Presentation]2013

    • Author(s)
      浅川義範, 中山維摩, 伊藤卓也
    • Organizer
      第57回香料・テルペンおよび精油化学に関する討論会
    • Place of Presentation
      埼玉
    • Related Report
      2013 Final Research Report
  • [Presentation] 放線菌由来新規化合物の探索2013

    • Author(s)
      山田陽香, 佐藤道大, 伊藤卓也, 高橋公咲, 野口博司, 渡辺賢二
    • Organizer
      第60回日本生薬学会
    • Place of Presentation
      北海道
    • Related Report
      2013 Final Research Report
  • [Presentation] α-グルコシダーゼ阻害物質トレスタチンの生合成遺伝子群のクローニング2013

    • Author(s)
      阿部高大, Taifo Mahmud, 兼目祐充, 浅川義範, 伊藤卓也
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      横浜
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] アンチマイシン生合成経路の探索と新規アナログの生産2013

    • Author(s)
      張魔騨, Yan YAN, 伊藤卓也, Xudong QU, 淡川孝義, 浅川義範, Wen Liu, 阿部郁朗
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      横浜
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] cryptolactone A及びその誘導体の合成と生物活性評価2013

    • Author(s)
      小栗友紀, 稲井誠, 伊藤卓也, 堀川美津代, 加来裕人, 角田鉄人
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      横浜
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 新規生物活性類縁体の創製を目指した放線菌代謝産物の生合成研究2012

    • Author(s)
      伊藤卓也
    • Organizer
      文科省戦略的研究基盤形成支援事業『疾患関連遺伝子及び疾患治療に用いる天然有機化合物の生合成遺伝子の包括的解析』第3回研究発表会
    • Place of Presentation
      徳島文理大学薬学部(徳島)
    • Year and Date
      2012-07-07
    • Related Report
      2013 Final Research Report
  • [Presentation] 新規生物活性類縁体の創製を目指した放線菌代謝産物の生合成研究2012

    • Author(s)
      伊藤卓也
    • Organizer
      第51回日本薬学会中国四国支部学術大会
    • Place of Presentation
      島根
    • Related Report
      2013 Final Research Report
  • [Presentation] Cloning and Characterization of Antimycin Biosynthetic Gene Cluster and Huge PKS Gene Cluster2012

    • Author(s)
      Ito, T,. Awakawa T., Qu, X., Yan, Y., Asakawa, Y., Liu, W., Abe, I.
    • Organizer
      International Conference of Natural Products Biosynthesis (ICNPB) Enzymology, Structural Biology, Drug Discovery and Genome Mining
    • Place of Presentation
      Awaji Shima, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] Calyculin A生合成遺伝子クラスターの探2012

    • Author(s)
      脇本敏幸, 江上蓉子, 淡川孝義, 伊藤卓也, 兼目裕允, 浅川義範, 阿部郁朗
    • Organizer
      第54回天然有機化合物討論会
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] シンビジウムグレートフラワー'マリーローランサン'の根の化学成分とそれらの抗菌活性について(2)2012

    • Author(s)
      吉川和子, 井関賀奈子, 伊藤卓也, 浅川義範, 橋本敏弘, 河野幸子
    • Organizer
      日本生薬学会第59回年会
    • Place of Presentation
      千葉
    • Related Report
      2013 Final Research Report
  • [Presentation] 新規生物活性類縁体の創製を目指した放線菌代謝産物の生合成研究2012

    • Author(s)
      伊藤卓也
    • Organizer
      第 51回日本薬学会中国四国支部学術大会
    • Place of Presentation
      松江
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Cloning and Characterization of Antimycin Biosynthetic Gene Cluster and Huge PKS Gene Cluster2012

    • Author(s)
      Takuya Ito, Takayoshi Awakawa, Xudong Qu, Yan Yan, Yoshinori Asakawa, Wen Liu, Ikuro Abe
    • Organizer
      International Conference of Natural Products Biosynthesis
    • Place of Presentation
      Awaji-shima
    • Related Report
      2012 Research-status Report
  • [Presentation] Calyculin A生合成遺伝子クラスターの探索2012

    • Author(s)
      脇本 敏幸、江上 蓉子、淡川 孝義、伊藤 卓也、兼目 裕允、浅川 義範、阿部 郁朗
    • Organizer
      第54回天然有機化合物討論会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] シンビジウム グレートフラワー • マリーローランサンの根の化学成分とそれらの抗菌活性について(2)2012

    • Author(s)
      吉川和子、井関賀奈子、伊藤卓也、浅川義範、橋本敏弘、河野幸子
    • Organizer
      日本生薬学会第59回年会
    • Place of Presentation
      千葉
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://p.bunri-u.ac.jp/lab01/home.htm

    • Related Report
      2013 Final Research Report
  • [Remarks] 徳島文理大学薬学部薬化学研究室

    • URL

      http://p.bunri-u.ac.jp/lab01/home.htm

    • Related Report
      2013 Annual Research Report
  • [Remarks] 文科省戦略的研究基盤形成支援事業 徳島文理大学大学院薬学研究科

    • URL

      http://p.bunri-u.ac.jp/lab04/senryaku/index.html

    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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