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Structure and dynamics measurements of thin filaments containing cardiomyopathy-causing mutant of troponin

Research Project

Project/Area Number 24710255
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Living organism molecular science
Research InstitutionJapan Atomic Energy Agency

Principal Investigator

MATSUO Tatsuhito  独立行政法人日本原子力研究開発機構, 原子力科学研究部門 量子ビーム応用研究センター, 研究員 (60623907)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsトロポニン / 中性子 / X線 / 心筋症 / 肥大型心筋症 / 小角散乱
Research Abstract

The effect of the cardiomyopathy-causing mutation in human cardiac troponin on its structure and dynamics was investigated. Troponin complex containing wild-type TnT2 or K247R mutant of TnT2 were reconstituted. Neutron scattering experiments on these solution samples have shown that the mutation causes the troponin molecule to be softer than the wild-type. The structural change of the native thin filament purified from a bovine heart upon calcium binding to TnC was detected by small-angle x-ray scattering (SAXS) experiments. The experiments to measure the structural change of the thin filament caused by the mutation of troponin are under way.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (5 results)

All 2013 2012

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (4 results)

  • [Journal Article] Dynamics of cardiomyopathy-causing mutant of troponin measured by neutron scattering2013

    • Author(s)
      Matsuo, T., Francesca, N., Plazanet M., Zaccai, G., Fujiwara, S.
    • Journal Title

      Journal of the Physical Society of Japan

      Volume: 82 Issue: Suppl.A Pages: 201-205

    • DOI

      10.7566/jpsjs.82sa.sa020

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Presentation] Characterization of the structural and dynamic properties of hydration water around F-actin detected by neutron and X-ray scattering2013

    • Author(s)
      Matsuo, T., Oda, T., Arata, T., Fujiwara, S.
    • Organizer
      Biophysical Society of Japan 51th annual meeting
    • Place of Presentation
      Kyoto
    • Year and Date
      2013-10-30
    • Related Report
      2013 Final Research Report
  • [Presentation] Characterization of the structural and dynamic properties of hydration water around F-actin detected by neutron and X-ray scattering2013

    • Author(s)
      Matsuo, T., Oda, T., Arata, T., Fujiwara, S.
    • Organizer
      Biophysical Society of Japan 51th annual meeting
    • Place of Presentation
      京都国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] Dynamics of cardiomyopathy-causing mutant of troponin measured by neutron scattering2012

    • Author(s)
      Matsuo T., Francesca, N., Plazanet M., Zaccai, G., Fujiwara, S.
    • Organizer
      10^<th> international conference on quasielastic neutron scattering and 5^<th> workshop on inelastic neutron spectrometers
    • Place of Presentation
      Nikko
    • Year and Date
      2012-10-02
    • Related Report
      2013 Final Research Report
  • [Presentation] Dynamics of cardiomyopathy-causing mutant of troponin measured by neutron scattering2012

    • Author(s)
      Tatsuhito Matsuo, Natali Francesca, Marie Plazanet, Giuseppe Zaccai, Satoru Fujiwara
    • Organizer
      10th international conference on quasielastic neutron scattering and 5th workshop on inelastic neutron spectrometers
    • Place of Presentation
      日光総合会館
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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