Structure and interaction analysis of GPI-related glycans

• Project/Area Number: 24710257
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Living organism molecular science
• Research Institution: Osaka University (2013-2014); The Institute of Physical and Chemical Research (2012)
• Principal Investigator: HANASHIMA Shinya 大阪大学, 理学(系)研究科(研究院), 講師 (50373353)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 糖鎖 / NMR / タンパク質 / 合成 / 相互作用 / ＮＭＲ

Outline of Final Research Achievements

We studied structures and a glycan-protein complex of functionally unknown GPI- related glycans and proteins through a combination of chemical and structural biological techniques. We chemically synthesized a series of truncated GPI-glycan structures. Enzymatic assay using the synthetic glycan units, human PGAP5, relates to GPI biological synthesis and ER-Golgi transport, requires full 5 sugar sequences for the substrate recognition. Our NMR study revealed that Mycobacterium phosphatidyl inositol glycan PIM is recognized by human lectin ZG16p which abundantly present around mucosa at intestine. Our finding suggests a new defense mechanism through mucosal immune responses.

Research Products

• [Journal Article] Defining the interaction of human soluble lectin ZG16p and mycobacterial phosphatidylinositol mannosides (2015)
  • Author(s): 1.Shinya Hanashima, Sebastian Götze, Yan Liu, Akemi Ikeda, Kyoko Kojima-Aikawa, Naoyuki Taniguchi, Daniel Varón Silva, Ten Feizi, Peter H. Seeberger, Yoshiki Yamaguchi
  • Journal Title: ChemBioChem Volume: 16 Issue: 10 Pages: 1502-11
  • DOI: 10.1002/cbic.201500103
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Synthesis of N-glycan units for assessment of substrate structural requirements of N-acetylglucosaminyltransferase III (2014)
  • Author(s): 2.Shinya Hanashima, Hiroaki Korekane, Naoyuki Taniguchi, Yoshiki Yamaguchi
  • Journal Title: Bioorganic and Medicinal Chemistry Letters Volume: 24 Issue: 18 Pages: 4533-4537
  • DOI: 10.1016/j.bmcl.2014.07.074
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] NMR study of short β(1-3)-glucans provides insights into the structure and interaction with Dectin-1. (2014)
  • Author(s): Hanashima S, Ikeda A, Tanaka H, Adachi Y, Ohno N, Takahashi T, Yamaguchi Y.
  • Journal Title: Glycoconjugate Journal Volume: 31 Issue: 3 Pages: 199-207
  • DOI: 10.1007/s10719-013-9510-x
  • Peer Reviewed
• [Presentation] バイセクト型糖鎖部分構造の合成と糖転移酵素阻害剤の分子設計戦略 (2014)
  • Author(s): 花島慎弥
  • Organizer: 第１２回糖鎖科学コンソーシアムシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2014-12-04 – 2014-12-05
  • Invited
• [Presentation] NMR interaction analysis of intestinal soluble lectin ZG16p with mycobacterium phosphatidylinositol mannosides (2014)
  • Author(s): Shinya Hanashima, Mayumi Kanagawa, Sebastian Götze, Yan Liu, Akemi Ikeda, Daniel Varón Silva, Ten Feizi, Peter Seeberger, Yoshiki Yamaguchi
  • Organizer: SFG2014 annual meeting
  • Place of Presentation: ハワイ
  • Year and Date: 2014-11-16 – 2014-11-19
• [Presentation] NMR analysis of Mycobacterium PIM glycans complexed with soluble lectin ZG16p (2014)
  • Author(s): 花島慎弥、Sebastian Götze、金川真由美、Yan Liu、池田明美、Ten Feizi、Peter Seeberger、谷口直之、山口芳樹
  • Organizer: 第33回日本糖質学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2014-08-10 – 2014-08-12
• [Presentation] NMR study of short β1,3-glucan structure and insight into the interaction with Dectin-1 (2013)
  • Author(s): Shinya Hanashima, Akemi Ikeda, Yoshiki Yamaguchi
  • Organizer: The 5th ACGG conference
  • Place of Presentation: Khon Kaen, Thailand
• [Presentation] NMRを用いた短鎖β1,3-glucanの構造と Dectin-1との相互作用解析 (2013)
  • Author(s): 花島慎弥
  • Organizer: 日本糖質学会年会
  • Place of Presentation: 大阪