| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Through the analysis of the molecular interaction between KRAP and IP3Rs, KRAP homolog gene was identified. KRAP homolog protein was specifically expressed in T- and B-lymphocytes and was found to interact with IP3Rs. KRAP homolog protein was localized around microdomain between ER and mitochondrial membranes, and appeared to regulate calcium ion flow from ER to mitochondria. It was worthy to note that both KRAP homolog and KRAP were phosphorylated upon extracellular calcium ion influx in lymphocytes and cancer cells, respectively. In this study, chemical screening to explore candidate of cancer drug targeting to KRAP-IP3R interaction was failed. However, elucidation of the molecular mechanisms underlying posttranslational modification of KRAP family proteins may provide a novel strategy for cancer theraphy.
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