Development of long-lived luminogenic probes for detection of DNA/RNA in a crude solution

• Project/Area Number: 24710266
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Chemical biology
• Research Institution: Kanagawa University (2013); The Institute of Physical and Chemical Research (2012)
• Principal Investigator: HISAO Saneyoshi 神奈川大学, 工学部, 助手 (10564784)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 核酸 / RNA / プローブ / 長寿命発光 / RNA検出 / 夾雑環境 / 長寿命発光発生型プローブ

Research Abstract

In this study, we developed chemical reaction triggered oligonucleotide probes that could detected DNA/RNA in a crude solution. Our probes have turn-on system that was induced by target DNA/RNA to produce long-lived luminessence. This method applied to the detection of RNA in living bacterial cells.

Research Products

• [Journal Article] Triphenylphosphinecarboxamide : An Effective Reagent for the Reduction of Azides and its Application to Nucleic Acid Detection (2014)
  • Author(s): Hisao Saneyoshi, Tatsuya Ochikubo, Takushi Mashimo, Ken Hatano, Yoshihiro Ito, Hiroshi Abe
  • Journal Title: Org lett Volume: 16 Pages: 30-33
  • Peer Reviewed
• [Journal Article] A Fluorogenic Peptide Probe Developed by In Vitro Selection Using tRNA Carrying a Fluorognic Amino Acid (2014)
  • Author(s): Wei Wang, Takanori Uzawa, Naoya Tochio, Jumpei Hamatsu, Yoshinori Hirano, Seiichi Tada, Hisao Saneyoshi, Takanori Kigawa, Nobuhiro Hayashi, Yutaka Ito, Makoto Taiji, Toshiro Aigaki, Yoshihiro Ito
  • Journal Title: Chem Comm Volume: 50 (22)
  • Peer Reviewed
• [Journal Article] Triphenylphosphinecarboxamide: An Effective Reagent for the Reduction of Azides and its Application to Nucleic Acid Detection (2014)
  • Author(s): Hisao Saneyoshi, Tatsuya Ochikubo, Takushi Mashimo, Ken Hatano, Yoshihiro Ito, Hiroshi Abe.
  • Journal Title: Org lett. Volume: 16 Issue: 1 Pages: 30-33
  • DOI: 10.1021/ol402832w
  • Peer Reviewed
• [Journal Article] Polycation-assisted DNA detection by reduction triggered fluorescence amplification probe (2013)
  • Author(s): Hisao Saneyoshi, Naohiko Shimada, Atsushi Maruyama, Yoshihiro Ito, Hiroshi Abe
  • Journal Title: Bioorg. Med Chem. Lett Volume: 23 Pages: 6851-6853
  • Peer Reviewed
• [Journal Article] Long-lived luminogenic probe for detection of RNA in a crude solution of living bacterial cells (2013)
  • Author(s): Hisao Saneyoshi, Yoshihiro Ito, Hiroshi Abe
  • Journal Title: J. Am. Chem. Soc Volume: 135
  • Peer Reviewed
• [Journal Article] Synthesis of a nucleoside phosphorodithioate analogue responsive to microenvironmental changes through chiral induction (2013)
  • Author(s): Hisao Saneyoshi, Takushi Mashimo, Ken Hatano, Yoshihiro Ito, Hiroshi Abe
  • Journal Title: Tetrahedron Lett Volume: 9 Pages: 1080-1083
  • Peer Reviewed
• [Journal Article] Long-lived luminogenic probe for detection of RNA in a crude solution of living bacterial cells. (2013)
  • Author(s): Hisao Saneyoshi, Yoshihiro Ito, Hiroshi Abe.
  • Journal Title: J. Am. Chem. Soc. Volume: 135 Issue: 37 Pages: 13632-13635
  • DOI: 10.1021/ja406724k
  • Peer Reviewed
• [Journal Article] Polycation-assisted DNA detection by reduction triggered fluorescence probe towards point-of-care testing (2013)
  • Author(s): H. Saneyoshi, N. Yoshida, N. Shimada, A. Maruyama, Y. Ito, H. Abe
  • Journal Title: Bioorg. Med. Chem. Volume: 23 Issue: 24 Pages: 6851-6853
  • DOI: 10.1016/j.bmcl.2013.10.005
  • Peer Reviewed
• [Presentation] 希土類発光プローブを用いた遺伝子検出プローブの開発 (2014)
  • Author(s): 阿部洋, 實吉尚郎, 周東智, 伊藤嘉浩
  • Organizer: 日本薬学会第134回年会
  • Place of Presentation: 熊本大学黒髪キャンパス
• [Presentation] 希土類発光プローブを用いた遺伝子検出プローブの開発 (2014)
  • Author(s): 阿部 洋、實吉尚郎、周東智、伊藤嘉浩
  • Organizer: 日本薬学会第134回年会
  • Place of Presentation: 熊本大学
• [Presentation] 隣接基関与を利用する還元反応の開発と核酸検出への応用 (2013)
  • Author(s): 實吉尚郎, 間下琢史, 幡野健, 阿部洋, 伊藤嘉浩
  • Organizer: 日本化学会第93春季年会
  • Place of Presentation: 立命館大学びわこくさつキャンパス
• [Presentation] 隣接基関与を利用する還元反応の開発と核酸検出への応用 (2013)
  • Author(s): 實吉尚郎、間下琢史、幡野健、阿部洋、伊藤嘉浩
  • Organizer: 日本化学会第93回春季年会
  • Place of Presentation: 立命館大学びわこ・くさつキャンパス
• [Presentation] Long lived luminogenic DNA probe for DNA/RNA detection (2012)
  • Author(s): Hisao Saneyoshi, Hiroshi Abe, Yoshihiro Ito
  • Organizer: 20 th International Round Table on Nucleosides, Nucleotides and Nucleic acids
  • Place of Presentation: Montréal
• [Presentation] Long lived luminogenic DNA probe for DNA/RNA detection (2012)
  • Author(s): Hisao Saneyoshi, Hiroshi Abe, Yoshihiro Ito
  • Organizer: 20 th International Round Table on Nucleosides, Nucleotides and Nucleic acids
  • Place of Presentation: カナダ モントリオール
• [Patent(Industrial Property Rights)] Fluorescent probe for detecting intracellular target mol (2012)
  • Inventor(s): Abe, Hiroshi, Hisao Saneyoshi, Ito, Yoshihiro
  • Industrial Property Rights Holder: Riken Corp., Japan
  • Industrial Property Rights Type: 特許
  • Acquisition Date: 2012-09-13
  • Overseas