| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Primula sieboldii, a perennial clonal herb, is distributed in Japan and eastern Asia. Overexploitation and destruction of its habitats and horticultural collection are threatening wild populations with extinction, and the species has become endangered in Japan. Seed production is limited in small populations in which the ratio of the two morphs (long-styled and short-styled) is imbalanced; this ratio is very important for reproductive success. The aim in this study was to develop molecular markers associated with the two morphs in a distylous wild population. These markers are likely to be useful tools for monitoring the ratio of morphs in a population containing the non-flowering ramets or during seasons when the ramets are not flowering. The result of RNA sequencing, specific single-nucleotide polymorphisms were detected in short-styled morph. Using these informations, developing molecular markers are aim for morph identification.
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