| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
Animal cells possess two types of actomyosin bundles called contractile rings and stress fibers, which are essential for cell division and cell shape maintenance, respectively. To investigate the assembly mechanisms of these cytoskeletal structures, we utilized purified actomyosin and cell-sized water-in-oil droplets or liposomes, and searched the biochemical and physical conditions at which the ordered structures resembling contractile rings and stress fibers were assembled. Although it was hard to verify the bistabilities of actomyosin systems, we found that contractile actomyosin rings were self-organized in a cell-sized spherically confined space, and contractile actomoysin bundle networks were self-organized when the motion of actin filaments was restricted in a two dimensional plane.
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