Design of fluorescent probes for siRNAs and their application to intracellular siRNA analysis
| Project/Area Number |
24750064
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Analytical chemistry
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| Research Institution | Tohoku University |
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Principal Investigator |
SATO YUSUKE 東北大学, 理学(系)研究科(研究院), 助教 (90583039)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 蛍光性分子 / siRNA / RNA干渉 / 蛍光イメージング / RNAi / 蛍光プローブ / PNA / インターカレーター |
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| Research Abstract |
We designed fluorescent probes for selective recognition of overhang nucleobases of siRNAs capable of inhibiting gene expression in cells. Our probes consist of peptide nucleic acid, pyrene and thiazole orange. The probes were found to exhibit the light-up fluorescence response upon selective binding to overhang nucleobases of siRNAs. Such a fluorescence sensing ability of the probe was applicable to distinct analysis of siRNA delivery through transfection. Then, this kind of fluorescence probe is expected to function as effective and versatile tools for the analysis of behaviors of intracellular siRNAs without replying on fluorophore modifications and labelling.
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Report
(3 results)
Research Products
(22 results)
