| Project/Area Number |
24750071
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Analytical chemistry
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| Research Institution | Kyushu Institute of Technology |
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Principal Investigator |
SATO Shinobu 九州工業大学, 工学(系)研究科(研究院), 准教授 (80510677)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | バイオセンサー / DNA / PCR / インターカレータ / 電気化学 / フェロセン / ナフタレンジイミド / シクロデキストリン / 電気化学遺伝子検出 / 核酸 |
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| Research Abstract |
Electrochemical DNA detection has been studying in light of health-care biochip and other related fields. It is also important to detect double stranded DNA amplified by PCR under homogeneous solution to check influenza type or norovirus. This can be achieved our developed detection system of double stranded DNA in homogenous solution. Ferrocenylnaphthalene diimide carrying beta-cyclodextrin (FNC) provides signal-on type electrochemical detection of double stranded DNA without DNA-immobilized electrode. FNC shows intramolecular inclusion complex between ferrocene and beta-cyclodextrin with diminished current signal. After bound to double stranded DNA, the complex is collapsed resulting in recovered current signal with positive shift of redox potential. One can detect PCR product electrochemically with high sensitivity under homogenous solution.
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