Development of an analytical system for the high sensitive detection of point mutation in oncogene
| Project/Area Number |
24750075
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Analytical chemistry
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
KOTANI Akira 東京薬科大学, 薬学部, 講師 (40318184)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 遺伝子多型 / 電気分析化学 / 変異検出法 / 液体クロマトグラフィー / 大腸癌 |
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| Research Abstract |
High-performance liquid chromatography with electrochemical detection (HPLC-ECD) using boron-doped diamond (BDD) electrode was developed to perform the mutation detection assay based on the ligase detection reaction (LDR), which can distinguish low-abundant mutant DNA from abundant wild-type DNA. The performance of the present HPLC-ECD was examined to separate and detect an oligonucleotide which was produced by LDR. By the present HPLC-ECD, the LDR product derived from KRAS codon 12.2 mutations was detected.
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Report
(3 results)
Research Products
(11 results)
