| Project/Area Number |
24750155
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemistry related to living body
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ASANUMA Daisuke 東京大学, 医学(系)研究科(研究院), 助教 (10611204)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 蛍光イメージング / 蛍光プローブ / 脳機能 / カルシウム / 神経活動 / カルシウムシグナル |
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| Research Abstract |
To reveal how neurons communicate with each other in neural networks of the brain, it is essential to develop imaging methods for selectively visualizing cellular activities in cell groups of interest. Here, I have constructed two such cell-type selective imaging methods by developing small-molecular fluorescence probes which are functionalized in target cells using genetic engineering techniques. (i) Method to visualize cell-selective calcium dynamics. I have developed calcium ion imaging probes which are functionalized by reporter enzyme and, using the optimal probe, have successfully visualized calcium dynamics in a cell-selective manner. (ii) Method to visualize synaptic transmission dynamics. I have developed acidic-pH-activatable probes which can monitor synaptic vesicle release by being conjugated to neural protein of interest using HaloTag labeling technology and, with the optimal probe, have successfully visualized neural activities.
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