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Functional molecular arrangements utilizing protein oligomerization by domain swapping

Research Project

Project/Area Number 24750163
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Chemistry related to living body
Research InstitutionNara Institute of Science and Technology

Principal Investigator

NAGAO Satoshi  奈良先端科学技術大学院大学, 物質創成科学研究科, 助教 (30452535)

Research Collaborator HIROTA Shun  奈良先端科学技術大学院大学, 物質創成科学研究科, 教授
KAMIKUBO Hironari  奈良先端科学技術大学院大学, 物質創成科学研究科, 准教授
TOMIOKA Yuya  
MOROI Maki  
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywordsタンパク質超分子 / 機能性分子 / ドメインスワッピング / 多量体 / シトクロムc / 多量化 / 光化学的特性 / ドメインスワップ / ミオグロビン / シトクロムc551 / 国際情報交換 / 中国 / インド
Outline of Final Research Achievements

Supramolecules have been known as the molecules assembled with the regulated intermolecular interactions. In this research, we tried to arrange functional molecules with protein oligomerization by domain swapping and investigated their structure and function.
We succeeded to construct zinc-substituted cytochrome c oligomers from monomers, and showed that the structural and photochemical properties did not change after oligomerization. We also showed that the carbon monoxide binding rate constant of dimeric myoglobin was about twice larger than that of the monomer, although the active site structure was similar between the monomer and dimer. We showed that cytochrome c551 formed dimers by domain swapping and retained the redox potential after dimerization. These results suggest that protein oligomerization by domain swapping can be useful to arrange functional molecules.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (12 results)

All 2015 2014 2013 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Acknowledgement Compliant: 3 results,  Open Access: 1 results) Presentation (7 results)

  • [Journal Article] Carbon monoxide binding properties of domain-swapped dimeric myoglobin2015

    • Author(s)
      Satoshi Nagao, Haruto Ishikawa, Takuya Yamada, Yasuhisa Mizutani, Shun Hirota
    • Journal Title

      Journal of biological inorganic chemistry

      Volume: 20 Issue: 3 Pages: 523-530

    • DOI

      10.1007/s00775-014-1236-0

    • NAID

      120005716870

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Domain-swapped dimer of Pseudomonas aeruginosa cytochrome c551: Structural insights into domain swapping of cytochrome c family proteins2015

    • Author(s)
      Satoshi Nagao, Mariko Ueda, Hisao Osuka, Hirofumi Komori, Hironari Kamikubo, Mikio Kataoka, Yoshiki Higuchi, Shun Hirota
    • Journal Title

      PLoS One

      Volume: 10 Issue: 4 Pages: e0123653-e0123653

    • DOI

      10.1371/journal.pone.0123653

    • NAID

      120005716860

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Rational design of heterodimeric protein using domain swapping for myoglobin2015

    • Author(s)
      Ying-Wu Lin, Satoshi Nagao, Mohan Zhang, Yasuhito Shomura, Yoshiki Higuchi, Shun Hirota
    • Journal Title

      Angewandte Chemie International Edition

      Volume: 54 Issue: 2 Pages: 511-515

    • DOI

      10.1002/anie.201409267

    • NAID

      120005716859

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article]2014

    • Author(s)
      S. Junedi, K. Yasuhara, S. Nagao, J. Kikuchi, S. Hirota
    • Journal Title

      ChemBioChem

      Volume: Vol. 15, No. 4 Issue: 4 Pages: 517-521

    • DOI

      10.1002/cbic.201300728

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Formation of Oligomeric Cytochrome c during Folding by lntermolecular Hydrophobic Interaction between N-and C-Terminal α-Helices2013

    • Author(s)
      Partha Pratim Parui, Megha Subhash Deshpande, Satoshi Nagao, Hironari Kamikubo. Hirofumi Komori. Yoshiki Higuchi, Miklo Kataoka . Shun Hirota
    • Journal Title

      Biochemistry

      Volume: 52巻 Issue: 48 Pages: 8732-8744

    • DOI

      10.1021/bi400986g

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Design and properties of domain-swapped dimeric myoglobin2015

    • Author(s)
      Satoshi Nagao, Ying-wu Lin, Haruto Ishikawa, Takuya Yamada, Mohan Zhang, Yasuhito Shomura, Yasuhisa Mizutani, Yoshiki Higuchi, Shun Hirota
    • Organizer
      日本化学会第95春季年会
    • Place of Presentation
      日本大学船橋キャンパス(千葉県船橋市)
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] Structural and ligand binding properties of dimeric horse myoglobin2014

    • Author(s)
      Satoshi Nagao, Hisao Osuka, Takuya Yamada, Takeshi Uni, Yasuhito Shomura, Kiyohiro Imai, Yoshiki Higuchi, Shun Hirota
    • Organizer
      The 28th Annual Symposium of the Protein Society
    • Place of Presentation
      San Diego (USA)
    • Year and Date
      2014-07-27 – 2014-07-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] シトクロムcとリン脂質二分子膜の相互作用に関する分光学的研究2014

    • Author(s)
      小林紀, 北田有希恵, 長尾聡, 廣田俊
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      愛知県名古屋市(名古屋大学)
    • Related Report
      2013 Research-status Report
  • [Presentation] 亜鉛置換ヘムタンパク質多量体の分子構造と光化学的性質2014

    • Author(s)
      長尾聡, 富岡勇也, 諸井麻希, 廣田俊
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      愛知県名古屋市(名古屋大学)
    • Related Report
      2013 Research-status Report
  • [Presentation] Binding of Oligomeric Cytochrome c to Cell Membrane2013

    • Author(s)
      S. Junedi, K. Yasuhara, S. Nagao, J. Kikuchi, S. Hirota
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      京都府京都市(京都国際会館)
    • Related Report
      2013 Research-status Report
  • [Presentation] ドメインスワッピングを利用した亜鉛置換ヘムタンパク質の多量体形成

    • Author(s)
      長尾聡、富岡勇也、諸井麻希、廣田俊
    • Organizer
      第6回バイオ関連化学シンポジウム
    • Place of Presentation
      北海道大学
    • Related Report
      2012 Research-status Report
  • [Presentation] ドメインスワッピングを利用した亜鉛置換シトクロムcの多量体形成

    • Author(s)
      長尾聡、富岡勇也、諸井麻希、廣田俊
    • Organizer
      日本化学会第93春季年会
    • Place of Presentation
      立命館大学
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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