| Project/Area Number |
24750167
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemistry related to living body
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| Research Institution | Konan University |
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Principal Investigator |
ENDOH Tamaki 甲南大学, 先端生命工学研究所, 講師 (90550236)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 転写共役構造形成 / RNA / 構造変化 / リボスイッチ / 準安定構造 / 最安定構造 / 低分子リガンド / 転写共役RNA構造形成 / 転写反応 / SELEX / 新生RNA構造形成 |
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| Research Abstract |
In this study, we functionalized RNA conformational switch for constructing biosensors and gene regulation systems by focusing on metastable RNA structure, which is formed by co-transcriptional folding during transcription. We selected RNAs, which show conformational transition in response to neomycin, from a library consists of neomycin aptamer and TAR-RNA connected by random nucleotides sequence. Then, based on a secondary structure of the selected RNA, we rationally designed RNA conformational switch that responds to thiamine pyrophosphate (TPP). The RNA enabled detection of TPP during transcription through co-transcriptional transition of nascent RNA structure. We also designed RNAs, which transition its structure from metastable secondary structure formed by co-transcriptional folding to most stable quadruplex structure. Intracellular gene expression depended on the rate of the conformation transition.
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