| Project/Area Number |
24750168
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemistry related to living body
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| Research Institution | Konan University |
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Principal Investigator |
TATEISHI Hisae 甲南大学, 先端生命研究所, 助教 (20593495)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 極限環境 / イオン液体 / 分子クラウディング / 核酸構造 / 熱力学的安定性 / 相互作用パラメータ / ミスマッチ塩基対 / ワトソン・クリック塩基対 |
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| Research Abstract |
We investigated the DNA and RNA behavior under ultimate environments such as ionic liquid and molecular crowding conditions.
We estimated the stability for DNA duplexes with Watson-Crick base pairs in a hydrated ionic liquid (IL) of choline dihydrogen phosphate (choline dhp), because hydrated ILs are green solvents and useful for nanotechnology. Our quantitative analysis demonstrated that A-T base pairs are more stable than G-C base pairs in the choline dhp. This is the reverse of base pair stabilities of DNA duplexes in buffered NaCl solution. Moreover, our molecular dynamics simulations revealed the mechanism underlying this difference. In contrast, we investigated the effect of molecular crowding induced by cosolutes on the stabilities of nucleic acids: Cosolutes change the water activity of solution and perturb the water binding to nucleic acids. The DNA and RNA behavior under ultimate environments will be useful in the design of oligonucleotides for use in nanomaterials.
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