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Mechanism for the signal transduction of CO-gas generated by heme oxygenase together with other proteins in the heme metabolism

Research Project

Project/Area Number 24750169
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Chemistry related to living body
Research InstitutionKurume University

Principal Investigator

HARADA Jiro  久留米大学, 医学部, 講師 (10373094)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywordsヘム酵素 / ヘムオキシゲナーゼ / NMR緩和分散測定 / ヘム代謝 / 国際情報交換
Outline of Final Research Achievements

Heme oxygenase catalyzes the degradation of heme. Heme oxygenase has been well characterized and revealed its heme binding mode in detail. In this study, we elucidated dynamics of Heme oxygenase by relaxation dispersion NMR spectroscopy, and detected structural fluctuation of a loop between C- and D-helices located on the surface. Thus far, the loop region has not been paid attention to, because it is located on the protein surface and far from the heme-binding site. Hence, we analyzed and characterized Leu77 and Phe79 which were the fluctuated amino acid residues in the loop.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (97 results)

All 2015 2014 2013 2012 Other

All Journal Article (17 results) (of which Peer Reviewed: 16 results,  Acknowledgement Compliant: 5 results,  Open Access: 4 results) Presentation (70 results) (of which Invited: 4 results) Remarks (7 results) Patent(Industrial Property Rights) (3 results) (of which Overseas: 1 results)

  • [Journal Article] Backbone assignments of the apo and Zn(II) protoporphyrin IX-bound states of the soluble form of rat heme oxygenase-12015

    • Author(s)
      Erisa Harada, Masakazu Sugishima, Jiro Harada, Masato Noguchi, Keiichi Fukuyama, Kenji Sugase
    • Journal Title

      Biomolecular NMR assignments

      Volume: 9(1) Issue: 1 Pages: 197-200

    • DOI

      10.1007/s12104-014-9573-z

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The 17-propionate esterifying variants of bacteriochlorophyll-a and bacteriopheophytin-a in purple photosynthetic bacteria2015

    • Author(s)
      T. Mizoguchi, M. Isaji, J. Harada, Y. Tsukatani, H. Tamiaki
    • Journal Title

      J. Photochem. Photobiol. B: Biol.

      Volume: 142 Pages: 244-249

    • DOI

      10.1016/j.jphotobiol.2014.12.013

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Distal regulation of heme binding of heme oxygenase-1 mediated by conformational fluctuations2015

    • Author(s)
      Erisa Harada, Masakazu Sugishima, Jiro Harada, Keiichi Fukuyama, Kenji Sugase
    • Journal Title

      Biochemistry

      Volume: 54(2) Issue: 2 Pages: 340-348

    • DOI

      10.1021/bi5009694

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Rhodobacter sphaeroides mutants overexpressing chlorophyllide a oxidoreductase of Blastochloris viridis elucidate functions of enzymes in late bacteriochlorophyll biosynthetic pathways2015

    • Author(s)
      Y. Tsukatani, J. Harada, J. Nomata, H. Yamamoto, Y. Fujita, T. Mizoguchi, H. Tamiaki
    • Journal Title

      Sci. Rep.

      Volume: 5 Issue: 1 Pages: 9741-9741

    • DOI

      10.1038/srep09741

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Chlorophyllide a oxidoreductase works as one of divinyl reductase specifically in bacteriochlorophyll a biosynthesis2014

    • Author(s)
      J. Harada, T. Mizoguchi, Y. Tsukatani, M. Yokono, A. Tanaka, H. Tamiaki
    • Journal Title

      J. Biol. Chem.

      Volume: 289 Issue: 18 Pages: 12716-12726

    • DOI

      10.1074/jbc.m113.546739

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Isolation and characterization of a new bacteriochlorophyll-c bearing a neopentyl substituent at the C8-position from the bciD-deletion mutant of the brown-colored green sulfur bacterium Chlorobaculum limnaeum2014

    • Author(s)
      T. Mizoguchi, J. Harada, Y. Tsukatani, H. Tamiaki
    • Journal Title

      Photosynth. Res.

      Volume: 121 Issue: 1 Pages: 3-12

    • DOI

      10.1007/s11120-014-9977-8

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Isolation and structural determination of C8-vinyl-bacteriochlorophyll d from the bciA and bchU double mutant of the green sulfur bacterium Chlorobaculum tepidum2014

    • Author(s)
      J. Harada, T. Mizoguchi, K. Nomura, H. Tamiaki
    • Journal Title

      Photosynth. Res.

      Volume: 121 Issue: 1 Pages: 13-23

    • DOI

      10.1007/s11120-014-0007-7

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Structural basis for the electron transfer from an open form of NADPH-cytochrome P450 oxidoreductase to heme oxygenase2014

    • Author(s)
      M. Sugishima, H. Sato, Y. Higashimoto, J. Harada, K. Wada, K. Fukuyama, M. Noguchi
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 111(7) Issue: 7 Pages: 2524-2529

    • DOI

      10.1073/pnas.1322034111

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Specific gene bciD for C7-methyl oxidation in bacteriochlorophyll e biosynthesis of brown-colored green sulfur bacteria2013

    • Author(s)
      J. Harada, T. Mizoguchi, S. Satoh, Y. Tsukatani, M. Yokono, M. Noguchi, A. Tanaka, H. Tamiaki
    • Journal Title

      PLoS ONE

      Volume: 8 Issue: 4 Pages: 60026-60026

    • DOI

      10.1371/journal.pone.0060026

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cyclopropane-ring formation in the acyl group of chlorosomal glycolipids is crucial for acid resistance of green bacterial antenna systems2013

    • Author(s)
      T. Mizoguchi, Y. Tsukatani, J. Harada, S. Takasaki, T. Yoshitomi, H. Tamiaki
    • Journal Title

      Bioorg. Med. Chem.

      Volume: 21 Issue: 13 Pages: 3689-3694

    • DOI

      10.1016/j.bmc.2013.04.030

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Bacteriochlorophyll homolog composition in the bchU mutants of green sulfur bacteria2013

    • Author(s)
      Y. Tsukatani, J. Harada, T. Mizoguchi, H. Tamiaki
    • Journal Title

      Photochem. Photobiol. Sci

      Volume: 12 Issue: 12 Pages: 2195-2201

    • DOI

      10.1039/c3pp50253h

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Gene expression system in the green sulfur bacterium Cholrobaculum tepidum by conjugative plasmid transfer2013

    • Author(s)
      C. Azai, J. Harada, H. Oh-oka
    • Journal Title

      PLoS ONE

      Volume: 8(11) Issue: 11 Pages: e82345-e82345

    • DOI

      10.1371/journal.pone.0082345

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] An unexpectedly branched biosynthetic pathway for bacteriochlorophyll b capable of absorbing near-infrared light2013

    • Author(s)
      Yusuke Tsukatani, Haruki Yamamoto, Jiro Harada, Taichi Yoshitomi, Jiro Nomata, Masahiro Kasahara, Tadashi Mizoguchi, Yuichi Fujita, and Hitoshi Tamiaki
    • Journal Title

      Scientific Reports

      Volume: 3 Issue: 1 Pages: 1217-1217

    • DOI

      10.1038/srep01217

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] A variety of glycolipids in green photosynthetic bacteria2013

    • Author(s)
      T. Mizoguchi
    • Journal Title

      Photosynth. Res.

      Volume: 114 Issue: 3 Pages: 179-188

    • DOI

      10.1007/s11120-013-9802-9

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] ついに発見!幻のクロロフィル -最も効率的な光合成アンテナ系で働く光捕集色素分子-.2013

    • Author(s)
      原田二朗、民秋 均
    • Journal Title

      月刊化学

      Volume: 68 Pages: 48-53

    • NAID

      40019592555

    • Related Report
      2012 Research-status Report
  • [Journal Article] A seventh bacterial chlorophyll driving a large light-harvesting antenna2012

    • Author(s)
      J. Harada
    • Journal Title

      Scientific Reports

      Volume: 2 Issue: 1 Pages: 671-671

    • DOI

      10.1038/srep00671

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characterization of chlorophyll pigments in the mutant lacking 8-vinyl reductase of green photosynthetic bacterium Chlorobaculum tepidum.2012

    • Author(s)
      T. Mizoguchi, J. Harada, and H. Tamiaki
    • Journal Title

      Bioorganic & Medicinal Chemistry

      Volume: 20 Pages: 6803-6810

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 緑色イオウ光合成細菌Chlorobaculum limnaeum RK-j-1株の変異体から単離精製した色素の異なるクロロソームの比較評価.2015

    • Author(s)
      原田二朗、柴田 穣、溝口 正、漁野 岬、寺村美里、山本 健、民秋 均
    • Organizer
      日本化学会第95春季年会
    • Place of Presentation
      船橋
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 緑色硫黄光合成細菌で働くクロロフィル色素の生合成に関与する2つの水和化酵素の異なる反応性.2015

    • Author(s)
      寺村美里、原田二朗、塚谷祐介、溝口 正、民秋 均
    • Organizer
      日本化学会第95春季年会
    • Place of Presentation
      船橋
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 緑色光合成細菌からのクロロゾームおよびFMOタンパク質の単離と人工光合成システムへの応用.2015

    • Author(s)
      漁野 岬、原田二朗、塚谷祐介、溝口 正、民秋 均
    • Organizer
      日本化学会第95春季年会
    • Place of Presentation
      船橋
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 緑色硫黄光合成細菌のバクテリオクロロフィルaシンターゼの酵素活性:基質と反応条件の影響.2015

    • Author(s)
      佐賀佳央、廣田圭耶、原田二朗、民秋 均
    • Organizer
      日本化学会第95春季年会
    • Place of Presentation
      船橋
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 好感度クロロフィル計測システムの開発と緑色光合成細菌の単一細胞測定への応用.2015

    • Author(s)
      溝口 正、伊佐治 恵、安井嘉秀、正津大介、軍司昌秀、原田二朗、塚谷祐介、民秋 均
    • Organizer
      日本化学会第95春季年会
    • Place of Presentation
      船橋
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 緑色硫黄細菌のバクテリオクロロフィル合成に関わる2つの水和化酵素BchFとBchVの生体内での役割.2015

    • Author(s)
      原田二朗、寺村美里、溝口 正、塚谷祐介、山本 健、民秋 均
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 様々な緑色光合成細菌から単離したクロロゾームとFMOタンパク質の比較と人工光合成系に向けた再構成系の検討.2015

    • Author(s)
      漁野 岬、原田二朗、塚谷祐介、溝口 正、民秋 均
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 超高感度クロロフィル計測システムの開発と緑色光合成細菌の単一細胞測定への応用.2015

    • Author(s)
      溝口 正、伊佐治 恵、安井嘉秀、正津大介、軍司昌秀、原田二朗、塚谷祐介、民秋 均
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] X線結晶構造解析によるヘム代謝酵素複合体の立体構造解明.2014

    • Author(s)
      杉島正一、佐藤秀明、東元祐一郎、原田二朗、平 順一、坂本 寛、安永卓生、和田 啓、福山恵一、山本 健、野口正人
    • Organizer
      立体視プロジェクションシステムを使った分子科学研究講演会
    • Place of Presentation
      飯塚
    • Year and Date
      2014-12-05 – 2014-12-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] Characterization of chlorosomes containing bacteriochlorophyll c, d, e, or f in the mutants derived from the identical strain of brown-colored green sulfur bacterium.2014

    • Author(s)
      J. Harada, Y. Shibata, M. Ryono, K. Yamamoto, T. Mizoguchi, and H. Tamiaki
    • Organizer
      2014 International Conference on Artificial Photosynthesis
    • Place of Presentation
      淡路
    • Year and Date
      2014-11-24 – 2014-11-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] C3^1 stereochemistry of bacteriochlorophyll c on light harvesting antenna of Chlorobaculum tepidum.2014

    • Author(s)
      M. Teramura, J. Harada, T. Mizoguchi, Y. Tsukatani, and H. Tamiaki
    • Organizer
      2014 International Conference on Artificial Photosynthesis
    • Place of Presentation
      淡路
    • Year and Date
      2014-11-24 – 2014-11-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] Isolation of various chlorosomes and FMO proteins toward construction of the artificial photosynthetic system.2014

    • Author(s)
      M. Ryono, J. Harada, Y. Tsukatani, T. Mizoguchi, and H. Tamiaki
    • Organizer
      2014 International Conference on Artificial Photosynthesis
    • Place of Presentation
      淡路
    • Year and Date
      2014-11-24 – 2014-11-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] Light-dependent changes of the composition related to bacteriochlorophyll-e homologs and its epimers.2014

    • Author(s)
      C. Okada, T. Mizoguchi, J. Harada, and H. Tamiaki
    • Organizer
      2014 International Conference on Artificial Photosynthesis
    • Place of Presentation
      淡路
    • Year and Date
      2014-11-24 – 2014-11-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] NADPH-シトクロムP450還元酵素とヘム-ヘムオキシゲナーゼ複合体との相互作用および電子伝達機構.2014

    • Author(s)
      杉島正一、小松将太、坂本 寛、安永卓夫、佐藤秀明、東元祐一郎、原田二朗、和田 啓、福山恵一、山本 健、野口正人
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 緑色硫黄光合成細菌の非モデル生物Chlorobaculum limnaeumでの形質転換法の確立.2014

    • Author(s)
      原田二朗、溝口 正、山本 健、民秋 均
    • Organizer
      第8回バイオ関連化学シンポジウム
    • Place of Presentation
      岡山
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] 緑色硫黄光合成細菌のバクテリオクロロフィル c生合成における立体選択的水和反応に関与する酵素遺伝子とその発現タンパク質の働き.2014

    • Author(s)
      寺村美里、原田二朗、溝口 正、塚谷祐介、民秋 均
    • Organizer
      第8回バイオ関連化学シンポジウム
    • Place of Presentation
      岡山
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] In-vitro stereospecific hydration of the C3-vinyl group of chlorophyll derivatives by recombinant enzymes.2014

    • Author(s)
      M. Teramura, J. Harada, T. Mizoguchi, Y. Tsukatani, and H. Tamiaki
    • Organizer
      The Photosynthesis Gordon Research Seminar
    • Place of Presentation
      USA・バーモント
    • Year and Date
      2014-08-10 – 2014-08-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] Stereospecific hydration of the C3-vinyl group involved in the bacteriochlorophylls biosynthesis of the photosynthetic green sulfur bacterium Chlorobaculum tepidum.2014

    • Author(s)
      M. Teramura, J. Harada, T. Mizoguchi, Y. Tsukatani, and H. Tamiaki
    • Organizer
      The photosynthesis Gordon Research Conference
    • Place of Presentation
      USA・バーモント
    • Year and Date
      2014-08-10 – 2014-08-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] Complex structure of NADPH-cytochrome P450 reductase and heme oxygenase-1.2014

    • Author(s)
      M. Sugishima, H. Sato, Y. Higashimoto, J. Harada, K. Wada, K. Fukuyama, and M. Noguchi
    • Organizer
      The 23rd International Union of Crystallography (IUCr) Congress and General Assembly
    • Place of Presentation
      カナダ・モントリーオール
    • Year and Date
      2014-08-05 – 2014-08-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] 3種類存在するC8位ビニル還元酵素(DVR)から考察されるクロロフィル色素合成系の分子進化.2014

    • Author(s)
      原田二朗、溝口 正、塚谷祐介、横野牧生、山本 健、田中 歩、民秋 均
    • Organizer
      第22回「光合成セミナー2014: 反応中心と色素系の多様性」
    • Place of Presentation
      名古屋
    • Year and Date
      2014-07-12 – 2014-07-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] 緑色硫黄光合成細菌が持つ2種類の3-ビニルヒドラターゼによるin vitroでの立体選択的水和反応.2014

    • Author(s)
      寺村美里、原田二朗、溝口 正、塚谷祐介、民秋 均
    • Organizer
      第22回「光合成セミナー2014: 反応中心と色素系の多様性」
    • Place of Presentation
      名古屋
    • Year and Date
      2014-07-12 – 2014-07-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] NADPH-シトクロムP450還元酵素からヘムオキシゲナーゼへの電子移動に関する構造基盤.2014

    • Author(s)
      杉島正一、東元祐一郎、佐藤秀明、原田二朗、和田 啓、福山恵一、野口正人
    • Organizer
      第14回日本蛋白質科学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-06-25 – 2014-06-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Chlorophyllide a oxidoreductase catalyzes the formation of an ethylidene group of bacteriochlorophyll b.2014

    • Author(s)
      Y. Tsukatani, H. Yamamoto, J. Harada, J. Nomata, T. Mizoguchi, Y. Fujita, and H. Tamiaki
    • Organizer
      Eighth International Conference on Porphyrins and Phthalocyanines
    • Place of Presentation
      トルコ・イスタンブール
    • Year and Date
      2014-06-22 – 2014-06-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] NADPH-シトクロムP450還元酵素とヘムオキシゲナーゼの複合体構造.2014

    • Author(s)
      杉島正一、東元祐一郎、佐藤秀明、原田二朗、坂本 寛、安永卓夫、和田 啓、福山恵一、野口正人
    • Organizer
      第41回生体分子科学討論会2014
    • Place of Presentation
      福岡
    • Year and Date
      2014-06-06 – 2014-06-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] In vitro enzymatic hydration of the 3-vinyl group in zinc chlorophyll derivatives.2014

    • Author(s)
      M. Teramura, J. Harada, T. Mizoguchi, Y. Tsukatani, and H. Tamiaki
    • Organizer
      Tenth International Workshop in Supramolecular Nanoscience of Chemically Programmed Pigments
    • Place of Presentation
      草津
    • Year and Date
      2014-05-30 – 2014-06-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] 3番目のC8位ビニル還元酵素(DVR)として同定されたクロロフィリド a酸化還元酵素(COR)の研究から考察されるクロロフィル合成の進化.2014

    • Author(s)
      原田二朗、溝口 正、塚谷祐介、横野牧生、田中 歩、民秋 均
    • Organizer
      第5回光合成研究会年会および公開シンポジウム
    • Place of Presentation
      奈良
    • Year and Date
      2014-05-30 – 2014-05-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] 紅色光合成細菌による「クロロフィル合成」.2014

    • Author(s)
      塚谷祐介、原田二朗、溝口 正、民秋 均、増田真二
    • Organizer
      第5回光合成研究会年会および公開シンポジウム
    • Place of Presentation
      奈良
    • Year and Date
      2014-05-30 – 2014-05-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] NADPH-シトクロムP450還元酵素からヘム-ヘムオキシゲナーゼの電子伝達に関する構造基盤.2014

    • Author(s)
      杉島正一、東元祐一郎、佐藤秀明、原田二朗、和田 啓、福山恵一、野口正人
    • Organizer
      平成26年度日本生化学会九州支部例会
    • Place of Presentation
      福岡
    • Year and Date
      2014-05-17 – 2014-05-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 緑色硫黄光合成細菌の巨大アンテナ系で働く色素の C31位を水酸化する2つの酵素は、その立体化学に大きく関与する.2014

    • Author(s)
      原田二朗,寺村里美,溝口 正,塚谷祐介,民秋 均
    • Organizer
      第16回生命化学研究会
    • Place of Presentation
      KKRホテル熱海,熱海市
    • Related Report
      2013 Research-status Report
  • [Presentation] Chlorophyllide a還元酵素で新たに見出されたC8位ビニル還元活性の進化的意義.2014

    • Author(s)
      原田二朗,溝口 正,塚谷祐介,横野牧生,田中 歩,民秋 均
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学五福キャンパス,富山市
    • Related Report
      2013 Research-status Report
  • [Presentation] クロロフィリドa還元酵素の導入による赤外光吸収型色素の遺伝子工学的生産.2014

    • Author(s)
      塚谷祐介,原田二朗,野亦次郎,溝口 正,藤田祐一,民秋 均
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学五福キャンパス,富山市
    • Related Report
      2013 Research-status Report
  • [Presentation] 緑色硫黄細菌のバクテリオクロロフィルc生合成における酵素遺伝子bchFとbchVの関与したC3位の立体選択的水和反応.2014

    • Author(s)
      溝口 正,寺村里美,原田二朗,塚谷祐介,民秋 均
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学五福キャンパス,富山市
    • Related Report
      2013 Research-status Report
  • [Presentation] ヘムオキシゲナーゼの基質ポケットから離れた分子表面のLeu77は、ビリベルジンとの親和性に関与する.2014

    • Author(s)
      原田二朗,佐藤秀明,杉島正一,原田英里砂,東元祐一郎,菅瀬謙治,野口正人
    • Organizer
      日本化学会第94回春季年会
    • Place of Presentation
      名古屋大学,名古屋市
    • Related Report
      2013 Research-status Report
  • [Presentation] クロロフィル色素合成における3-ビニルヒドロラターゼのin vitroでの反応.2014

    • Author(s)
      民秋 均,寺村里美,溝口 正,塚谷祐介,原田二朗
    • Organizer
      日本化学会第94回春季年会
    • Place of Presentation
      名古屋大学,名古屋市
    • Related Report
      2013 Research-status Report
  • [Presentation] 色素合成酵素遺伝子bciDはバクテリオクロロフィルeのC7位のホルミル化に関与する.2013

    • Author(s)
      原田二朗,溝口 正,佐藤壮一郎,塚谷祐介,横野牧生,野口正人,田中 歩,民秋 均
    • Organizer
      第4回光合成研究会年会
    • Place of Presentation
      名古屋大学,名古屋市
    • Related Report
      2013 Research-status Report
  • [Presentation] 緑色流黄細菌の光捕集体クロロソームの脂質分解:阻害剤添加および変異体による解析.2013

    • Author(s)
      塚谷祐介,溝口 正,原田二朗,民秋 均
    • Organizer
      第4回光合成研究会年会
    • Place of Presentation
      名古屋大学,名古屋市
    • Related Report
      2013 Research-status Report
  • [Presentation] A variety of composite bacteriochlorophyll pigments in purple photosynthetic bacteria.2013

    • Author(s)
      T. Mizoguchi, M. Isaji, J. Harada, Y. Tsukatani, and H. Tamiaki
    • Organizer
      9th International Workshop in Supramolecular Nanoscience of Chemically Programmed Pigments
    • Place of Presentation
      立命館大学,草津市
    • Related Report
      2013 Research-status Report
  • [Presentation] Bacteriochlorophyll homolog composition analyses in mutants of green sulfur bacteria.2013

    • Author(s)
      Y. Tsukatani, J. Harada, T. Mizoguchi, and H. Tamiaki
    • Organizer
      9th International Workshop in Supramolecular Nanoscience of Chemically Programmed Pigments
    • Place of Presentation
      立命館大学,草津市
    • Related Report
      2013 Research-status Report
  • [Presentation] 同一生体から精製したバクテリオクロロフィルc、eおよびfをそれぞれもつクロロソームの比較.2013

    • Author(s)
      原田二朗,溝口 正,柴田 穣,塚谷祐介,野口正人,民秋 均
    • Organizer
      第21回光合成セミナー
    • Place of Presentation
      名古屋工業大学,名古屋市
    • Related Report
      2013 Research-status Report
  • [Presentation] Chlorosomes containing bacteriochlorophyll c, e, or f in the brown-colored green sulfur bacterium Chlorobaculum limnaeum.2013

    • Author(s)
      J. Harada, T. Mizoguchi, Y. Tsukatani, M. Noguchi, and H. Tamiaki
    • Organizer
      Light-Harvesting Satellite Meeting 2013
    • Place of Presentation
      ワシントン大学,セントルイス, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] Chlorophyllode a oxidoreductase branches biosynthetic pathways for bacteriochlorophylls a and b.2013

    • Author(s)
      Y. Tsukatani, H. Yamamoto, J. Harada, J. Nomata, T. Mizoguchi, Y. Fujita, and H. Tamiaki
    • Organizer
      Light-Harvesting Satellite Meeting 2013
    • Place of Presentation
      ワシントン大学,セントルイス, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] Bacteriochlorophyll e biosynthetic pathway of the brown-colored green sulfur bacterium Chlorobaculum limnaeum.2013

    • Author(s)
      J. Harada, T. Mizoguchi, S. Satoh, Y. Tsukatani, M. Yokono, M. Noguchi, A. Tanaka, and H. Tamiaki
    • Organizer
      The 16th International congress on photosynthesis research
    • Place of Presentation
      Hyatt Regency at The Arch,セントルイス, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] Hinge 欠損型 NADPH-シトクロムP450還元酵素とヘムオキシゲナーゼ間の相互作用.2013

    • Author(s)
      杉島正一,東元祐一郎,佐藤秀明,原田二朗,下川千寿,城田沙織,野口正人
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜,横浜市
    • Related Report
      2013 Research-status Report
  • [Presentation] Ala置換変異によってヘムオキシゲナーゼ活性が上昇するLeu77の機能解析.2013

    • Author(s)
      原田二朗,佐藤秀明,原田英里砂,東元祐一郎,杉島正一,菅瀬謙治,野口正人
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜,横浜市
    • Related Report
      2013 Research-status Report
  • [Presentation] 緑色硫黄光合成細菌のバクテリオクロロフィル合成酵素の活性測定.2013

    • Author(s)
      佐賀佳央,廣田圭耶,原田二朗,民秋 均
    • Organizer
      第7回バイオ関連化学合同シンポジウム2013
    • Place of Presentation
      名古屋大学,名古屋市
    • Related Report
      2013 Research-status Report
  • [Presentation] NADPH-シトクロムP450還元酵素-ヘム-ヘムオキシゲナーゼ複合体の結晶構造解析に向けて.2013

    • Author(s)
      杉島正一,東元祐一郎,佐藤秀明,原田二朗,野口正人
    • Organizer
      平成25年度日本結晶学会年会
    • Place of Presentation
      熊本大学,熊本市
    • Related Report
      2013 Research-status Report
  • [Presentation] lasticity of chlorophyllide a oxidoreductase, a versatile nitrogenase-like enzyme.2013

    • Author(s)
      Y. Tsukatani, H. Yamamoto, J. Harada, J. Nomata, T. Mizoguchi, Y. Fujita, and H. Tamiaki
    • Organizer
      18th Intertanional Congress on Nitrogen Fixation
    • Place of Presentation
      Phenix Seagaia resort, 宮崎市
    • Related Report
      2013 Research-status Report
  • [Presentation] 緑色硫黄細菌のバクテリオクロロフィル eのC7位のホルミル化に必須な遺伝子bciDの同定.2013

    • Author(s)
      原田二朗、溝口 正、佐藤壮一郎、塚谷祐介、横野牧生、野口正人、田中 歩、民秋 均
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山県・岡山
    • Related Report
      2012 Research-status Report
  • [Presentation] クロロフィリド a還元酵素によるバクテリオクロロフィル bのエチリデン基形成.2013

    • Author(s)
      塚谷祐介、山本治樹、原田二朗、吉冨太一、溝口 正、藤田祐一、民秋 均
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山県・岡山
    • Related Report
      2012 Research-status Report
  • [Presentation] ヘムオキシゲナーゼ分子表面で揺らぐLeu77のAla置換変異体は何故酵素活性を上昇させるのか?2013

    • Author(s)
      原田二朗、佐藤秀明、原田英里砂、東元祐一郎、杉島正一、菅瀬謙治、野口正人
    • Organizer
      日本化学会第93回春季年会
    • Place of Presentation
      滋賀県・草津
    • Related Report
      2012 Research-status Report
  • [Presentation] NMR解析で見出されたヘムオキシゲナーゼ表面に局在するF79の機能的役割.2012

    • Author(s)
      原田二朗、原田英里砂、東元祐一郎、杉島正一、佐藤秀明、平 順一、福山恵一、菅瀬謙治、野口正人
    • Organizer
      平成24年度日本生化学会九州支部例会
    • Place of Presentation
      福岡県・福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] The role of amino acids on the surface of heme oxygenase in its catalysis: An analysis of F79A mutant.”, 7th International Congress on Heme Oxygenase and Rated Enzymes2012

    • Author(s)
      J. Harada, E. Harada, Y. Higashimoto, H. Sato, M. Sugishima, J. Taira, K. Fukuyama, K. Sugase, and M. Noguchi
    • Organizer
      7th International Congress on Heme Oxygenase and Rated Enzymes
    • Place of Presentation
      イギリス・エジンバラ
    • Related Report
      2012 Research-status Report
  • [Presentation] Reduction of CO-bound α-verdoheme-heme oxygenase-1 complex by NADPH-cytochrome P450 reductase.2012

    • Author(s)
      H. Sato, Y. Higashimoto, H. Sakamoto, M. Sugishima, J. Harada, and M. Noguchi
    • Organizer
      7th International Congress on Heme Oxygenase and Rated Enzymes
    • Place of Presentation
      イギリス・エジンバラ
    • Related Report
      2012 Research-status Report
  • [Presentation] Molecular biological approach on biosynthesis of bacteriochlorophylls in phototrophic bacteria.2012

    • Author(s)
      Y. Tsukatani, J. Harada, and H. Tamiaki
    • Organizer
      Eight International Workshop in Supramolecular Nanoscience of Chemically Programmed Pigments
    • Place of Presentation
      滋賀県・草津
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] The Structure and Function of Glycolipids in Photosynthesis.2012

    • Author(s)
      T. Mizoguchi, J. Harada, T. Yoshitomi, and H. Tamiaki
    • Organizer
      Eight International Workshop in Supramolecular Nanoscience of Chemically Programmed Pigments
    • Place of Presentation
      滋賀県・草津
    • Related Report
      2012 Research-status Report
  • [Presentation] 新規色素バクテリオクロロフィル fは緑色硫黄細菌の生体内でアンテナ系クロロソームを駆動する.2012

    • Author(s)
      原田二朗、溝口 正、塚谷祐介、野口正人、民秋 均
    • Organizer
      第20回光合成の色素系と反応中心に関するセミナー
    • Place of Presentation
      大阪府・豊中
    • Related Report
      2012 Research-status Report
  • [Presentation] 緑色硫黄細菌Chlorobaculum tepidumのC8位ビニル還元酵素遺伝子変異体の色素組成から考察されるバクテリオクロロフィル生合成経路.2012

    • Author(s)
      原田二朗、溝口 正、吉冨太一、野口正人、民秋 均
    • Organizer
      第20回光合成の色素系と反応中心に関するセミナー
    • Place of Presentation
      大阪府・豊中
    • Related Report
      2012 Research-status Report
  • [Presentation] バクテリオクロロフィルb生合成経路の解明.2012

    • Author(s)
      塚谷祐介、山本治樹、原田二朗、吉冨太一、溝口 正、藤田祐一、民秋 均
    • Organizer
      第20回光合成の色素系と反応中心に関するセミナー
    • Place of Presentation
      大阪府・豊中
    • Related Report
      2012 Research-status Report
  • [Presentation] 種々の緑色硫黄細菌の網羅的糖脂質解析.2012

    • Author(s)
      溝口 正、原田二朗、吉冨太一、民秋 均
    • Organizer
      第20回光合成の色素系と反応中心に関するセミナー
    • Place of Presentation
      大阪府・豊中
    • Related Report
      2012 Research-status Report
  • [Presentation] Characterization of the novel monogalactosyldiacylglycerol (MGDG) synthase, found in green sulfur bacteria, reveals mechanisms of chlorosome biogenesis.2012

    • Author(s)
      S. Masuda, J. Harada, M. Noguchi, and H. Ohta
    • Organizer
      14th International Symposium on Phototrophic Prokaryotes
    • Place of Presentation
      ポルトガル・ポルト
    • Related Report
      2012 Research-status Report
  • [Presentation] Studies on the reduction processes from chlorophyll a estrified with geranylgeraniol to that with Δ2,6-phytadienol in the green sulfur bacterium Chlorobaculum tepidum.2012

    • Author(s)
      H. Oh-oka, C. Azai, J. Harada, S. Miyago, T. Mizoguchi, and H. Tamiaki
    • Organizer
      14th International Symposium on Phototrophic Prokaryotes
    • Place of Presentation
      ポルトガル・ポルト
    • Related Report
      2012 Research-status Report
  • [Presentation] Role of structural fluctuation in enzyme catalysis of heme oxygenase 1.2012

    • Author(s)
      E. Harada, M. Sugishima, J. Harada, M. Noguchi, K. Fukuyama, and K. Sugase
    • Organizer
      25th International conference on Magnetic resonance in biological systems
    • Place of Presentation
      フランス・リヨン
    • Related Report
      2012 Research-status Report
  • [Presentation] ヘム結合時にヘムオキシゲナーゼの分子表面で揺らぐアミノ酸残基F79の変異体解析から見出される知見.2012

    • Author(s)
      原田二朗、原田英里砂、東元祐一郎、杉島正一、佐藤秀明、平 順一、福山恵一、菅瀬謙治、野口正人
    • Organizer
      第6回バイオ関連化学合同シンポジウム2012
    • Place of Presentation
      北海道・札幌
    • Related Report
      2012 Research-status Report
  • [Presentation] クロロフィル色素のC17位上に結合する炭化水素鎖の生合成過程.2012

    • Author(s)
      原田二朗、溝口 正、民秋 均、大岡宏造
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      兵庫県・姫路
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] ヘムオキシゲナーゼの温度依存的構造変化と酵素反応に与える影響.2012

    • Author(s)
      杉島正一、原田二朗、東元 裕一郎、Keith Moffat、野口正人
    • Organizer
      平成24年度日本結晶学会年会及び総会
    • Place of Presentation
      宮城県・仙台
    • Related Report
      2012 Research-status Report
  • [Presentation] Genetic analysis of the green sulfur bacterium Chlorobaculum limnaeum.2012

    • Author(s)
      J. Harada, T. Mizoguchi, Y. Tsukatani, M. Noguchi, and H. Tamiaki
    • Organizer
      Second International Symposium on Biosynthesis of Tetrapyrroles
    • Place of Presentation
      滋賀県・草津
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Chlorophyllode a oxidoreductase, important nitrogenase-like enzymes with versatile functions.2012

    • Author(s)
      Y. Tsukatani, H. Yamamoto, J. Harada, T. Mizoguchi, Y. Fujita, and H. Tamiaki
    • Organizer
      Second International Symposium on Biosynthesis of Tetrapyrroles
    • Place of Presentation
      滋賀県・草津
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 揺らぎが制御するヘム分解酵素反応の遠位効果.2012

    • Author(s)
      原田英里砂、杉島正一、原田二朗、野口正人、福山恵一、菅瀬謙治
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡県・福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] ヘムオキシゲナーゼ分子表面に存在するF79の変異導入解析から見出された電子伝達機構.2012

    • Author(s)
      原田二朗、原田英里砂、東元祐一郎、杉島正一、佐藤秀明、平 順一、福山恵一、菅瀬謙治、野口正人
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡県・福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] ニトロゲナーゼ類似酵素であるクロロフィリド a還元酵素によるバクテリオクロロフィル bのエチリデン基形成.2012

    • Author(s)
      塚谷祐介、山本治樹、原田二朗、吉冨太一、溝口 正、藤田祐一、民秋 均
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡県・福岡
    • Related Report
      2012 Research-status Report
  • [Remarks] 久留米大学研究者紹介 医学部医化学講座 原田二朗

    • URL

      http://research.kurume-u.ac.jp/data.php?scode=82055632889065

    • Related Report
      2014 Annual Research Report
  • [Remarks] 久留米大学研究者紹介:原田二朗

    • URL

      http://research.kurume-u.ac.jp/data.php?scode=82055632889065

    • Related Report
      2013 Research-status Report
  • [Remarks] Google Scholar Citations: Jiro Harada

    • URL

      http://scholar.google.com/citations?user=M61fT_QAAAAJ&hl=en

    • Related Report
      2013 Research-status Report
  • [Remarks] ウェブ報道, 「ヘム鉄代謝の鍵を握る伝達物質の立体構造の解明に成功」, マイナビニュース

    • URL

      http://news.mynavi.jp/news/2014/02/05/083/

    • Related Report
      2013 Research-status Report
  • [Remarks] ウェブ報道, マイナビニュース

    • URL

      http://news.mynavi.jp/news/2012/09/28/151/index.html

    • Related Report
      2012 Research-status Report
  • [Remarks] ウェブ報道, 大学プレスセンター

    • URL

      http://artificial-photosynthesis.net/katsudo/media.html

    • Related Report
      2012 Research-status Report
  • [Remarks] ウェブ報道, 日経バイオテクONLINE

    • URL

      https://bio.nikkeibp.co.jp/article/news/20120928/163467/

    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] バクテリオクロロフィルbの大量産生方法及び産生菌.2014

    • Inventor(s)
      塚谷祐介,民秋 均,原田二朗,藤田祐一,野亦次郎
    • Industrial Property Rights Holder
      塚谷祐介,民秋 均,原田二朗,藤田祐一,野亦次郎
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-030085
    • Filing Date
      2014-02-19
    • Related Report
      2013 Research-status Report
  • [Patent(Industrial Property Rights)] 緑色硫黄細菌変異体およびバクテリオクロロフィル.2013

    • Inventor(s)
      原田二朗、野口正人、民秋 均
    • Industrial Property Rights Holder
      原田二朗、野口正人、民秋 均
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-02-13
    • Related Report
      2012 Research-status Report
    • Overseas
  • [Patent(Industrial Property Rights)] 緑色硫黄細菌変異株及びそれを用いたバクテリオクロロフィルc同族体の製造方法.2012

    • Inventor(s)
      原田二朗、野口正人、民秋 均
    • Industrial Property Rights Holder
      原田二朗、野口正人、民秋 均
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2012-260321
    • Filing Date
      2012-11-28
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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