Elucidation of the dynamic conformations and interactions of oligosaccharides by using paramagnetic NMR probes
| Project/Area Number |
24750170
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemistry related to living body
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| Research Institution | Institute for Molecular Science |
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Principal Investigator |
YAMAGUCHI Takumi 分子科学研究所, 生命・錯体分子科学研究領域, 助教 (60522430)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 糖鎖 / NMR / 常磁性 / 立体構造解析 / ダイナミクス |
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| Outline of Final Research Achievements |
Elucidation of physicochemical basis of the biological functions of oligosaccharides remains as tasks with more challenges. Paramagnetism-assisted NMR techniques using lanthanide ion-chelating probes was combined with molecular dynamics simulations to explore the conformational spaces of oligosaccharides in solution. The experimentally validated simulation results successfully provided atomic descriptions of dynamic behaviors of oligosaccharides such as high-mannose-type oligosaccharides harboring intracellular glycoprotein-fate determinants in their branched structures. The data showed conformational selection mechanisms by the carbohydrate recognition proteins during the glycan recognition process. These results suggest that dynamic conformational rearrangements of oligosaccharides can be important factors in inspecting carbohydrate-binding affinities and specificities of the lectins and enzymes in cells.
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Report
(4 results)
Research Products
(40 results)
