Design and development of the fluorescent-labeled siRNA for studying RNAi
| Project/Area Number |
24750173
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemistry related to living body
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| Research Institution | Nagoya University |
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Principal Investigator |
KAMIYA Yukiko 名古屋大学, エコトピア科学研究所, 講師 (00527947)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | RNAi / RNA結合タンパク質 / siRNA / miRNA / 機能性核酸 / 蛍光プローブ / クロスリンク / RNA-タンパク相互作用 |
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| Research Abstract |
RNA interference (RNAi) is an endogenous gene silencing system in living cells. It has been utilized to knock down a specific gene by introducing the corresponding short double-stranded RNA (siRNA) into cells. In this research, we developed chemically modified siRNA with functional molecules including dyes or cross-linker. We found a novel design of siRNAs that are incorporated into RISC with high antisense strand selectivity without loss of RNAi activity. Using our designed fluorophore-quencher pair-conjugated siRNA, we successfully demonstrated selective monitoring of mature-RISC which embedded the antisense strand in cells.
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Report
(3 results)
Research Products
(29 results)
