| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Research Abstract |
RNA interference (RNAi) is an endogenous gene silencing system in living cells. It has been utilized to knock down a specific gene by introducing the corresponding short double-stranded RNA (siRNA) into cells. In this research, we developed chemically modified siRNA with functional molecules including dyes or cross-linker. We found a novel design of siRNAs that are incorporated into RISC with high antisense strand selectivity without loss of RNAi activity. Using our designed fluorophore-quencher pair-conjugated siRNA, we successfully demonstrated selective monitoring of mature-RISC which embedded the antisense strand in cells.
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