Three dimensional quantitative phase measurement system and its application to cytodiagnosis
| Project/Area Number |
24760038
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied optics/Quantum optical engineering
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| Research Institution | The University of Electro-Communications |
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Principal Investigator |
WATANABE Eriko 電気通信大学, 情報理工学研究科, 准教授 (20424765)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | ホログラフィ / 光計測 / 定量位相顕微鏡 / 細胞検査 / パターン認識 |
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| Outline of Final Research Achievements |
In this work, we developed two types of quantitative phase microscopes. One is a scan-type phase measurement system that does not require an phase unwrapping process. The other is the Digital Holographic Microscope (DHM), using a spherical reference beam that can measure wide field phase distribution. The scan-type phase measurement system is based on the Mach–Zehnder interferometer, which uses a phase-locking technique. In our preliminary experiments, cancer cells were distinguished from normal cells through comparison of differences in optical path length. The DHM system, which allows for high-precision quantitative phase measurements of large areas, facilitates improvements in terms of the diffraction calculation. With this system, we proposed a novel autofocusing technique that exploits the intensity distribution of the transparent phase object. We successfully obtained high-resolution large-area images of sheets of the cells.
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Report
(4 results)
Research Products
(25 results)
