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Relationship between intracellular mechanical field and mechanosensors in stretched cell

Research Project

Project/Area Number 24760078
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Materials/Mechanics of materials
Research InstitutionThe University of Tokushima

Principal Investigator

SATO Katsuya  徳島大学, ソシオテクノサイエンス研究部, 講師 (10403651)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords細胞力覚 / 骨芽細胞 / 伸展刺激 / 力学的刺激 / バイオメカニクス / MEMS
Research Abstract

In this study, we developed a novel cell stretching micro device to observe cellular response to stretching stimuli with high spatial and temporal resolution.
First, we have modified our stretching device to reduce the focus drift during the stretching. And we have introduced the fluorescent ratiometric measurement to reduce influence of change in fluorescent intensity arose from the focus drift. Finally, we succeed to conduct observation of cellular response to stretching stimuli with high spatial and temporal resolution.
Second, we developed an experimental system to evaluate the intracellular strain field in stretched cell. We combined our cell stretching micro device and the image correlation method to measure the strain in stretched cell. We succeed to evaluated the strain field in the cell during stretch.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (12 results)

All 2013 2012 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (8 results) Remarks (1 results)

  • [Journal Article] Myosin-II-mediated directional migration of dictyostelium cells in response to cyclic stretching of substratum2013

    • Author(s)
      Yoshiaki Iwadate, Chika Okimura, Katsuya Sato, Yuta Nakashima, Masatsune Tsujioka and Kazuyuki Minami
    • Journal Title

      Biophysical Journal

      Volume: Vol.104 Pages: 748-758

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of Highly Precise Cell Stretching Microdevice and in-situ Observation of Stretched Cell2013

    • Author(s)
      中島雄太,門司亮,佐藤克也,南和幸
    • Journal Title

      IEEJ Transactions on Sensors and Micromachines

      Volume: 133 Issue: 12 Pages: 350-357

    • DOI

      10.1541/ieejsmas.133.350

    • NAID

      130003382695

    • ISSN
      1341-8939, 1347-5525
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Myosin-II-mediated directional migration of dictyostelium cells in response to cyclic stretching of substratum2013

    • Author(s)
      Yoshiaki Iwadate, et al.
    • Journal Title

      Biophysical Journal

      Volume: 104 Issue: 4 Pages: 748-758

    • DOI

      10.1016/j.bpj.2013.01.005

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] ストレッチ刺激に対する培養骨芽細胞のカルシウム応答のその場観察2013

    • Author(s)
      佐藤克也, 他
    • Organizer
      第36回日本生体医工学会中国四国支部大会
    • Place of Presentation
      愛媛大学医学部(愛媛県)
    • Year and Date
      2013-10-19
    • Related Report
      2013 Final Research Report
  • [Presentation] 引張りを受ける骨芽細胞のカルシウム応答動態のその場観察2013

    • Author(s)
      佐藤克也, 他
    • Organizer
      日本機械学会第25回バイオエンジニアリング講演会
    • Place of Presentation
      産業技術総合研究所(茨城県)
    • Related Report
      2013 Final Research Report
  • [Presentation] ストレッチ刺激に対する培養骨芽細胞のカルシウム応答のその場観察2013

    • Author(s)
      佐藤克也,門司亮,中島雄太,南和幸,伊藤伸一,藤澤正一郎
    • Organizer
      第36回日本生体医工学会中国四国支部大会
    • Place of Presentation
      愛媛大学医学部(愛媛県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 引張りを受ける骨芽細胞のカルシウム応答動態のその場観察2013

    • Author(s)
      佐藤克也
    • Organizer
      日本機械学会第25回バイオエンジニアリング講演会
    • Place of Presentation
      産業技術総合研究所(茨城県)
    • Related Report
      2012 Research-status Report
  • [Presentation] 引張りひずみ負荷に対する骨芽細胞応答のその場観察と評価2012

    • Author(s)
      佐藤克也, 他
    • Organizer
      日本機械学会M&M2012材料力学カンファレンス
    • Place of Presentation
      愛媛大学(愛媛県)
    • Related Report
      2013 Final Research Report
  • [Presentation] ストレッチを受ける細胞のリアルタイムその場観察用マイクロデバイスの開発2012

    • Author(s)
      佐藤克也, 他
    • Organizer
      日本生体医工学会第51回大会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Related Report
      2013 Final Research Report
  • [Presentation] 引張りひずみ負荷に対する骨芽細胞応答のその場観察と評価2012

    • Author(s)
      佐藤克也
    • Organizer
      日本機械学会M&M2012 材料力学カンファレンス
    • Place of Presentation
      愛媛大学(愛媛県)
    • Related Report
      2012 Research-status Report
  • [Presentation] ストレッチを受ける細胞のリアルタイムその場観察用マイクロデバイスの開発2012

    • Author(s)
      佐藤克也
    • Organizer
      日本生体医工学会第51回大会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.me.tokushima-u.ac.jp/aaelab/

    • Related Report
      2013 Final Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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