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Three Dimensional Analysis of the Locomotion of Bacteria near a Surface

Research Project

Project/Area Number 24760136
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Fluid engineering
Research InstitutionTottori University

Principal Investigator

NAKAI Tonau  鳥取大学, 工学(系)研究科(研究院), 助教 (80452548)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords細菌 / 集団運動 / べん毛 / 3次元計測 / 顕微鏡 / 流体解析 / 画像処理 / 3次元観察 / 追跡 / 数値計算
Research Abstract

To find the mechanism of formation of bacterial biofilm, we observed 3 dimensional swimming motions of bacteria. We constructed the biplane microscopy that has two orthogonal optical axes. By analysis of the obtained images, 3D trajectories of bacterial swimming are measured. We also conducted measurement of the collective motion of bacteria. Bacillus subtilis cells swim collectively when the culture medium becomes crowded with the average distance less than 5 micrometers. To quantify the degree of collective motion, we compared the velocity correlation of swarmer cells with that of randomly swimming cells. Large difference is found at the distance of 10 micrometers, which is several times as long as the size of a cell.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (39 results)

All 2014 2013 2012 Other

All Journal Article (6 results) (of which Peer Reviewed: 3 results) Presentation (29 results) (of which Invited: 2 results) Remarks (3 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Boundary Element Analysis and Three-Dimensional Observation of Propulsive Force and Torque of a Dinoflagellate Symbiodinium2013

    • Author(s)
      Tonau Nakai, Ikuko Shihira-Ishikawa, Atsushi Miyawaki, Tomonobu Goto
    • Journal Title

      Journal of Aero Aqua Bio-mechanisms

      Volume: Vol. 3, No. 1 Pages: 79-84

    • NAID

      130003375384

    • URL

      https://www.jstage.jst.go.jp/article/jabmech/3/1/3_79/_article

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Bacterial Locomotion in an Infinite Liquid Medium and in the Presence of a Nearby Surface2013

    • Author(s)
      Tomonobu Goto, Tonau Nakai
    • Journal Title

      Journal of Aero Aqua Bio-mechanisms

      Volume: Vol. 3, No. 1 Pages: 2-7

    • NAID

      130003375373

    • URL

      https://www.jstage.jst.go.jp/article/jabmech/3/1/3_2/_article

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Dialysis Purification of Integrase-DNA Complexes Provides High-Resolution Atomic Force Microscopy Images: Dimeric Recombinant HIV-1 Integrase Binding and Specific Looping on DNA2013

    • Author(s)
      Tatsuaki Tsuruyama, Tonau Nakai, Rei Ohmori, Munetaka Ozeki, Keiji Tamaki, Kenichi Yoshikawa
    • Journal Title

      PLoS One

      Volume: 8 Issue: 1 Pages: e53572-e53572

    • DOI

      10.1371/journal.pone.0053572

    • NAID

      120005241337

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 大学生の理系文章作成能力の現状と改善に向けた取り組み2013

    • Author(s)
      中井唱、星健夫、吉本芳英
    • Journal Title

      鳥取大学 教育研究論集

      Volume: 3 Pages: 77-82

    • Related Report
      2012 Research-status Report
  • [Journal Article] 細菌の泳ぎと流体力学2012

    • Author(s)
      後藤知伸,中井唱
    • Journal Title

      油空圧技術

      Volume: 51 Pages: 38-44

    • Related Report
      2012 Research-status Report
  • [Journal Article] 細菌の泳ぎと流体力学

    • Author(s)
      後藤知伸,中井唱
    • Journal Title

      油空圧技術2012年7月号

      Volume: 637 Vol. 51, No. 7 Pages: 38-44

    • Related Report
      2013 Final Research Report
  • [Presentation] 二方向同時観察法を用いた微生物の三次元自動追跡2014

    • Author(s)
      植木裕太,中井唱,後藤知伸
    • Organizer
      日本機械学会中国四国支部第52期講演会
    • Place of Presentation
      鳥取大学
    • Year and Date
      2014-03-07
    • Related Report
      2013 Final Research Report
  • [Presentation] 細菌の集団運動による物質輸送の粒子画像解析2014

    • Author(s)
      小寺広起,中井唱,後藤知伸
    • Organizer
      日本機械学会中国四国学生会第44回学生員卒業研究発表講演会
    • Place of Presentation
      鳥取大学
    • Year and Date
      2014-03-06
    • Related Report
      2013 Final Research Report
  • [Presentation] 近接して回転する二本のらせん型べん毛の流体解析2014

    • Author(s)
      神田将志,中井唱,後藤知伸
    • Organizer
      日本機械学会中国四国学生会第44回学生員卒業研究発表講演会
    • Place of Presentation
      鳥取大学
    • Year and Date
      2014-03-06
    • Related Report
      2013 Final Research Report
  • [Presentation] 細菌の集団運動における細菌個体間の速度相関と数密度の関係2014

    • Author(s)
      毛利佳裕
    • Organizer
      日本機械学会ロボティクス・メカトロニクス講演会2014
    • Place of Presentation
      富山市総合体育館
    • Related Report
      2013 Annual Research Report
  • [Presentation] 2方向同時観察法を用いた遊泳微生物の3次元自動追跡2014

    • Author(s)
      中井唱
    • Organizer
      日本機械学会ロボティクス・メカトロニクス講演会2014
    • Place of Presentation
      富山市総合体育館
    • Related Report
      2013 Annual Research Report
  • [Presentation] Analysis of Velocity Correlation in the Collective Motion of Bacteria2014

    • Author(s)
      Tonau Nakai
    • Organizer
      7th World Congress of Biomechanics
    • Place of Presentation
      John B. Hynes Veterans Memorial Convention Center (Boston, U.S.A)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 二方向同時観察法を用いた微生物の三次元自動追跡2014

    • Author(s)
      植木裕太
    • Organizer
      日本機械学会中国四国支部第52期講演会
    • Place of Presentation
      鳥取大学湖山キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細菌の集団運動による物質輸送の粒子画像解析2014

    • Author(s)
      小寺広起
    • Organizer
      日本機械学会中国四国学生会第44回学生員卒業研究発表講演会
    • Place of Presentation
      鳥取大学湖山キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 近接して回転する二本のらせん型べん毛の流体解析2014

    • Author(s)
      神田将志
    • Organizer
      日本機械学会中国四国学生会第44回学生員卒業研究発表講演会
    • Place of Presentation
      鳥取大学湖山キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細菌の速度の解析による集団運動の定量化2013

    • Author(s)
      毛利佳裕,中井唱,後藤知伸
    • Organizer
      日本機械学会2013年度年次大会
    • Place of Presentation
      岡山大学
    • Year and Date
      2013-09-10
    • Related Report
      2013 Final Research Report
  • [Presentation] Analysis of Velocity Correlation in Bacterial Swarming2013

    • Author(s)
      Tonau NAKAI, Yoshihiro MOURI, Tomonobu GOTO
    • Organizer
      The 7th Asian Pacific Conference on Biomechanics
    • Place of Presentation
      KIST, Seoul, Korea
    • Year and Date
      2013-08-31
    • Related Report
      2013 Final Research Report
  • [Presentation] 同期した二方向映像の輝度変化に対応した細菌の三次元位置及び姿勢の自動推定2013

    • Author(s)
      三上和博,中井唱,後藤知伸
    • Organizer
      日本機械学会中国四国支部第51期講演会
    • Place of Presentation
      高知工科大学
    • Year and Date
      2013-03-08
    • Related Report
      2013 Final Research Report
  • [Presentation] 細菌の数密度と速度の解析による集団運動の定量化2013

    • Author(s)
      毛利佳裕
    • Organizer
      鳥取非線形研究会2013
    • Place of Presentation
      鳥取大学湖山キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 2方向顕微鏡観察法を用いた微生物運動の3次元解析2013

    • Author(s)
      中井唱
    • Organizer
      日本機械学会第24回バイオフロンティア講演会
    • Place of Presentation
      同志社大学今出川キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細菌の速度の解析による集団運動の定量化2013

    • Author(s)
      毛利佳裕
    • Organizer
      日本機械学会2013年度年次大会
    • Place of Presentation
      岡山大学津島キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] Analysis of Velocity Correlation in Bacterial Swarming2013

    • Author(s)
      Tonau Nakai
    • Organizer
      The 7th Asian Pacific Conference on Biomechanics
    • Place of Presentation
      KIST(Seoul, Korea)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細菌の集団運動(Swarming)の速度相関の計測2013

    • Author(s)
      毛利佳裕
    • Organizer
      日本機械学会ロボティクス・メカトロニクス講演会2013
    • Place of Presentation
      つくば国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細菌の速度の解析による集団運動の定量化2013

    • Author(s)
      毛利佳裕
    • Organizer
      日本機械学会年次大会
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Research-status Report
  • [Presentation] 細菌の集団運動(Swarming)の速度相関の計測2013

    • Author(s)
      毛利佳裕
    • Organizer
      日本機械学会 ロボティクス・メカトロニクス部門講演会
    • Place of Presentation
      つくば国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] 同期した二方向映像の輝度変化に対応した細菌の三次元位置及び姿勢の自動推定2013

    • Author(s)
      三上和博
    • Organizer
      日本機械学会 中国四国支部 第51期講演会
    • Place of Presentation
      高知工科大学
    • Related Report
      2012 Research-status Report
  • [Presentation] 細菌の集団運動(Swarming)の定量化2013

    • Author(s)
      毛利佳裕
    • Organizer
      日本機械学会 中国四国学生会 第43回学生員卒業研究発表講演会
    • Place of Presentation
      高知工科大学
    • Related Report
      2012 Research-status Report
  • [Presentation] らせん型べん毛により推進する細菌の推進効率に関する数値解析2013

    • Author(s)
      三宅正芳
    • Organizer
      日本機械学会第25回バイオエンジニアリング講演会
    • Place of Presentation
      産業技術総合研究所(つくば)
    • Related Report
      2012 Research-status Report
  • [Presentation] BOUNDARY ELEMENT ANALYSIS OF PROPULSIVE FORCE AND TORQUE OF A DINOFLAGELLATE SYMBIODINIUM2012

    • Author(s)
      Tonau Nakai, Ikuko Shihira-Ishikawa, Atsushi Miyawaki, Tomonobu Goto
    • Organizer
      The 23rd International Congress of Theoretical and Applied Mechanics (ICTAM2012)
    • Place of Presentation
      China National Convention Center北京,中国
    • Year and Date
      2012-08-20
    • Related Report
      2013 Final Research Report
  • [Presentation] 二方向同時観察法を用いた三次元自動追跡機能付き顕微鏡の試作(二次元平面内でのリアルタイム自動追跡)2012

    • Author(s)
      植木裕太
    • Organizer
      日本機械学会 2012年度年次大会
    • Place of Presentation
      金沢大学
    • Related Report
      2012 Research-status Report
  • [Presentation] Boundary Element Analysis and Three-Dimensional Observation of Propulsive Force and Torque of a Dinoflagellate Symbiodinium2012

    • Author(s)
      Tonau Nakai
    • Organizer
      The Fifth International Symposium on Aero Aqua Bio-Mechanisms
    • Place of Presentation
      Howard International House (Taipei)
    • Related Report
      2012 Research-status Report
  • [Presentation] Bacterial Locomotion in an Infinite Liquid Medium and in the Presence of a Nearby Surface2012

    • Author(s)
      Tomonobu Goto
    • Organizer
      The Fifth International Symposium on Aero Aqua Bio-Mechanisms
    • Place of Presentation
      Howard International House (Taipei)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] BOUNDARY ELEMENT ANALYSIS OF PROPULSIVE FORCE AND TORQUE OF A DINOFLAGELLATE SYMBIODINIUM2012

    • Author(s)
      Tonau Nakai
    • Organizer
      The 23rd International Congress of Theoretical and Applied Mechanics (ICTAM2012)
    • Place of Presentation
      China National Convention Center
    • Related Report
      2012 Research-status Report
  • [Presentation] 推進するらせん型の形状最適化に関するストークス流れの数値解析2012

    • Author(s)
      三宅正芳
    • Organizer
      日本機械学会 ロボティクス・メカトロニクス講演会2012
    • Place of Presentation
      アクトシティ浜松
    • Related Report
      2012 Research-status Report
  • [Presentation] 2方向の明視野顕微鏡像を用いた細菌の3次元位置及び姿勢の自動推定2012

    • Author(s)
      三上和博
    • Organizer
      日本機械学会 ロボティクス・メカトロニクス講演会2012
    • Place of Presentation
      アクトシティ浜松
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.damp.tottori-u.ac.jp/~lab5/index.html

    • Related Report
      2013 Final Research Report
  • [Remarks] 生体システム解析学研究室

    • URL

      http://www.damp.tottori-u.ac.jp/~lab5/index.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] 生体システム解析学研究室

    • URL

      http://www.damp.tottori-u.ac.jp/~lab5/index.html

    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] 三次元顕微鏡装置及び同装置を用いた観察・測定法2013

    • Inventor(s)
      後藤 知伸、中井 唱、菊田 誠之
    • Industrial Property Rights Holder
      国立大学法人鳥取大学
    • Industrial Property Rights Type
      特許
    • Acquisition Date
      2013-06-28
    • Related Report
      2013 Final Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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