Occurrence of Campylobacter in water environment and structure analysis of Campylobacter antigen associated with Guillain-Barré syndrome

• Project/Area Number: 24760431
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Civil and environmental engineering
• Research Institution: Kyoto University
• Principal Investigator: ASADA Yasuhiro 京都大学, 工学(系)研究科(研究院), 助教 (60610524)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 健康関連微生物 / 上水道工学 / リスク評価 / カンピロバクター / ギラン・バレー症候群 / リポオリゴ糖 / シアル酸 / Campylobacter jejuni / 免疫磁気ビーズ / MPN-PCR

Research Abstract

The numbers of Campylobacter jejuni in water source were measured. C. jejuni in water source contains 0.011-1.5 MPN/L and is frequently abundant in summer and winter. Next, C. jejuni associated with GBS in water source was surveyed. The presence of sialic acid on C. jejuni lipooligosaccharide (LOS) is considered a risk factor for developing GBS. The LOS extracts of isolated C. jejuni were analyzed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). C. jejuni with sialylated LOS was regarded as the C. jejuni associated with GBS. Some C. jejuni strains isolated from water source had sialylated LOS. These C. jejuni strains were considered as C. jejuni associated with GBS.

Research Products

• [Journal Article] 糖鎖構造解析に基づいた河川水中Campylobacter jejuniのシアル酸含有リポオリゴ糖保有実態 (2013)
  • Author(s): 浅田安廣,大河内由美子,越後信哉,伊藤禎彦
  • Journal Title: 環境衛生工学研究 Volume: Vol.27, No.3 Pages: 208-211
• [Journal Article] 糖鎖構造解析に基づいた河川水中Campylobacter jejuniのシアル酸含有リポオリゴ糖保有実態 (2013)
  • Author(s): 浅田安廣，大河内由美子，越後信哉，伊藤禎彦
  • Journal Title: 環境衛生工学研究 Volume: Vol.27 Pages: 208-211
• [Journal Article] 環境水中のCampylobacter jejuni存在実態解明に向けた分離および定量手法の確立 (2012)
  • Author(s): 浅田安廣,大河内由美子,伊藤禎彦
  • Journal Title: 環境衛生工学研究 Volume: Vol.26, No.3 Pages: 144-147
• [Journal Article] 環境水中のCampylobacter jejuni存在実態解明に向けた分離および定量手法の確立 (2012)
  • Author(s): 浅田安廣、大河内由美子、伊藤禎彦
  • Journal Title: 環境衛生工学研究 Volume: Vol.26 Pages: 144-147
• [Presentation] 環境水中に存在するCampylobacter jejuniの定量における増菌培養法の比較 (2013)
  • Author(s): 浅田安廣,大河内由美子,伊藤禎彦
  • Organizer: 第47回日本水環境学会年会
  • Place of Presentation: 大阪工業大学大宮キャンパス
• [Presentation] Investigation of methods for isolation and quantitation of Campylobacter jejuni in water environment (2013)
  • Author(s): Asada, Y., Ohkouchi, Y. and Itoh, S.
  • Organizer: 22^<th> KKNN symposium
  • Place of Presentation: 韓国・ソウル
• [Presentation] Structure Analysis of Campylobacter jejuni Lipooligosaccharisde Associated with Guillain-Barre Syndrome in Source Water For Estimating Disability Adjusted Life Years (2013)
  • Author(s): Asada, Y., Ohkouchi, Y., Matudate, K., Echigo, S. and Itoh, S.
  • Organizer: The 17^<th> Internetional Symposium on Health-Related Water Microbiology
  • Place of Presentation: ブラジル・フロリアノポリス
• [Presentation] 障害調整生存年数推定を目的とした河川水中Campylobacter jejuniの実態調査 (2013)
  • Author(s): 浅田安廣,大河内由美子,松館圭太,伊藤禎彦
  • Place of Presentation: 琉球大学
• [Presentation] 河川水中Campylobacter jejuniに対するギラン・バレー症候群発症関連菌株の推定 (2013)
  • Author(s): 浅田安廣,大河内由美子,越後信哉,伊藤禎彦
  • Place of Presentation: 北海道大学
• [Presentation] Structure analysis of Campylobacter jejuni lipooligosaccharide associated with Guillain-Barre' syndrome in source water for estimating disability adjusted life years (2013)
  • Author(s): Asada, Y, Ohkouchi Y, Matudate K, Echigo S and Itoh S
  • Organizer: The 17th Internetional Symposium on Health-Related Water Microbiology
  • Place of Presentation: Florianopolis, Brazil
• [Presentation] 障害調整生存年数推定を目的とした河川水中Campylobacter jejuniの実態調査 (2013)
  • Author(s): 浅田安廣、大河内由美子、松館圭太、伊藤禎彦
  • Organizer: 第16回日本水環境学会シンポジウム
  • Place of Presentation: 琉球大学，沖縄県
• [Presentation] 河川水中Campylobacter jejuniに対するギラン・バレー症候群発症関連菌株の推定 (2013)
  • Author(s): 浅田安廣、大河内由美子、越後信哉、伊藤禎彦
  • Organizer: 第50回環境工学研究フォーラム
  • Place of Presentation: 北海道大学，北海道
• [Presentation] Investigation of methods for isolation and quantitation of Campylobacter jejuni in water environment (2013)
  • Author(s): Yasuhiro Asada
  • Organizer: 22st KAIST-KU-NTU-NUS Symposium on Environmental Engineering
  • Place of Presentation: Seoul, Korea
• [Presentation] 環境水中に存在するCampylobacter jejuniに対する分離方法の探索ならびに血清型調査 (2012)
  • Author(s): 浅田安廣,大河内由美子,伊藤禎彦
  • Organizer: 第5回日本カンピロバクター研究会
  • Place of Presentation: 大阪府立りんくうキャンパス
• [Presentation] 環境水中に存在するCampylobacter jejuniに対する分離方法の探索ならびに血清型調査 (2012)
  • Author(s): 浅田安廣
  • Organizer: 第5回日本カンピロバクター研究会
  • Place of Presentation: 大阪府立大学りんくうキャンパス