Occurrence of Campylobacter in water environment and structure analysis of Campylobacter antigen associated with Guillain-Barré syndrome
| Project/Area Number |
24760431
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Civil and environmental engineering
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| Research Institution | Kyoto University |
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Principal Investigator |
ASADA Yasuhiro 京都大学, 工学(系)研究科(研究院), 助教 (60610524)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 健康関連微生物 / 上水道工学 / リスク評価 / カンピロバクター / ギラン・バレー症候群 / リポオリゴ糖 / シアル酸 / Campylobacter jejuni / 免疫磁気ビーズ / MPN-PCR |
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| Research Abstract |
The numbers of Campylobacter jejuni in water source were measured. C. jejuni in water source contains 0.011-1.5 MPN/L and is frequently abundant in summer and winter. Next, C. jejuni associated with GBS in water source was surveyed. The presence of sialic acid on C. jejuni lipooligosaccharide (LOS) is considered a risk factor for developing GBS. The LOS extracts of isolated C. jejuni were analyzed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). C. jejuni with sialylated LOS was regarded as the C. jejuni associated with GBS. Some C. jejuni strains isolated from water source had sialylated LOS. These C. jejuni strains were considered as C. jejuni associated with GBS.
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Report
(3 results)
Research Products
(15 results)
