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Ecophysiology of the uncultured microorganism reside in anaerobic sewage treatment process

Research Project

Project/Area Number 24760433
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Civil and environmental engineering
Research InstitutionNigata University of Phermacy and Applied Life Sciences

Principal Investigator

IGUCHI Akinori  新潟薬科大学, 応用生物科学部, 助教 (60599786)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords未培養微生物 / 下水処理 / メタン発酵プロセス / 微生物群集構造解析 / 途上国 / UASB
Outline of Final Research Achievements

Functions of the uncultured OP5 microorganisms frequently detected in sewage treatment methane fermentation processes were analyzed. The results of spatial distribution of the uncultured OP5 microorganism in sludge granules with fluorescence in situ hybridization and substrate estimation experiment based on rRNA expression, OP5 microorganisms may utilize organic acid (i.e. acetate and propionate) while symbiosis with hydrogen utilizing archaea. The result of attempting to isolate the uncultured OP5 microorganism, the OP5 microorganism and hydrogen utilizing archaea could be enriched with acetate as carbon source.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (20 results)

All 2015 2014 2013 2012 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 1 results,  Open Access: 1 results) Presentation (15 results) Remarks (1 results)

  • [Journal Article] 実証規模DHSリアクターの処理性能と硝化細菌群に及ぼすスポンジ担体の影響2014

    • Author(s)
      大久保努, 井口晃徳, 久保田健吾, 山口隆司, 上村繁樹, 原田秀樹.
    • Journal Title

      下水道協会誌

      Volume: 51 Pages: 121-128

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Microbial community composition of a down-flow hanging sponge (DHS) reactor combined with an up-flow anaerobic sludge blanket (UASB) reactor for the treatment of municipal sewage.2014

    • Author(s)
      Kengo Kubota, Mikio Hayashi, Kengo Matsunaga, Akinori Iguchi, Akiyoshi Ohashi, Yu-You Li, Takashi Yamaguchi, Hideki Harada
    • Journal Title

      Bioresource Technology

      Volume: 151 Pages: 144-150

    • DOI

      10.1016/j.biortech.2013.10.058

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Microbial community composition of a down-flow hanging sponge (DHS) reactor combined with an up-flow anaerobic sludge blanket (UASB) reactor for the treatment of municipal sewage.2014

    • Author(s)
      K. Kubota, M. Hayashi, K. Matsunaga, A. Iguchi, A. Ohashi, Y.Y. Li, T. Yamaguchi, H. Harada.
    • Journal Title

      Bioresource Technology

      Volume: 151 Pages: 144-155

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] 実証規模UASB-DHSシステムにおける後段DHSリアクターの微生物群集構造解析と脱窒細菌群の定量.2013

    • Author(s)
      井口晃徳,大久保努,立花真,永井寛之,上村繁樹,山口隆司,久保田健吾,原田秀樹.
    • Journal Title

      土木学会論文集G (環境)

      Volume: 69 Pages: 539-546

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 下水の灌漑利用における病原性微生物を対象とした定量的微生物リスク評価2015

    • Author(s)
      内田翔太,大久保努,上村繁樹,荒木信夫,多川正,井口晃徳,高橋優信,久保田健吾,原田秀樹
    • Organizer
      第49回日本水環境学会年会
    • Place of Presentation
      金沢
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 下水処理UASB汚泥から高頻度に検出される未培養, 微生物の基質利用推定2015

    • Author(s)
      5-1 千葉有紀,佐藤江美,井口晃徳,林真由美,山口隆司, 原田秀樹, 重松亨
    • Organizer
      第49回日本水環境学会年会
    • Place of Presentation
      金沢
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 最初沈澱池-DHSシステムを用いた灌漑再利用のための都市下水処理2014

    • Author(s)
      瀬戸雄太, 多川正, 竹村泰幸, 久保田健吾, 井口晃徳, 大久保努, 上村繁樹, 高橋優信, 久保田健吾, 原田秀樹
    • Organizer
      第51回土木学会環境工学研究委員会 環境工学研究フォーラム
    • Place of Presentation
      山梨
    • Year and Date
      2014-12-20 – 2014-12-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] 定量的微生物リスク評価に基づいた下水の灌漑利用時における複合感染リスクの評価2014

    • Author(s)
      内田翔太, 大久保努, 上村繁樹, 荒木信夫, 多川正, 井口晃徳, 高橋優信, 久保田健吾, 原田 秀樹
    • Organizer
      第51回土木学会環境工学研究委員会 環境工学研究フォーラム
    • Place of Presentation
      山梨
    • Year and Date
      2014-12-20 – 2014-12-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] 下水処理嫌気性汚泥から高頻度に検出される未培養微生物の基質利用推定2014

    • Author(s)
      千葉有紀, 林真由美, 佐藤江美, 井口晃徳, 重松 亨
    • Organizer
      第32回土木学会関東支部新潟会研究調査発表会
    • Place of Presentation
      長岡
    • Year and Date
      2014-11-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] In situ ecophysiological analysis of uncultured Caldiserica phylotype reside in UASB sludge granules treating sewage.2014

    • Author(s)
      Yuki Chiba, Akinori IGUCHI, Toru Shigematsu, Masashi Hatamoto, Takashi Yamaguchi, Masanobu Takahashi, Kengo Kubota, Hideki Harada.
    • Organizer
      International Workshop on “UASB-DHS integrated system – a sustainable sewage treatment technology”
    • Place of Presentation
      Delhi, India
    • Year and Date
      2014-10-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] Application of DHS technology for sewage treatment and microbiology there.2014

    • Author(s)
      Kengo Kubota, Tadashi Tagawa,Yuta Seto, Akihiro Nagamachi, Akinori Iguchi, Shigeki Uemura, Tsutomu Okubo, Masanobu Takahashi, and Hideki Harada
    • Organizer
      International Workshop on “ UASB-DHS integrated system – a sustainable sewage treatment technology”
    • Place of Presentation
      Delhi, India
    • Year and Date
      2014-10-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] Evaluation of a novel sewage treatment system by combining a primary settled + DHS reactor2014

    • Author(s)
      Akihiro Nagamachi, Yuta Seto, Tadashi Tagawa, Tsutomu Okubo, Shigeki Uemura, Nobuo Araki, Akinori Iguchi, Kengo Kubota, Masanobu Takahashi and Hideki Harada
    • Organizer
      International Workshop on “ UASB-DHS integrated system – a sustainable sewage treatment technology”
    • Place of Presentation
      Delhi, India
    • Year and Date
      2014-10-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] ファージディスプレイ法を利用した特定微生物検出・回収技術のハイスループット化の検討2014

    • Author(s)
      井口晃徳, 佐々木波輝, 原田秀樹, 重松亨
    • Organizer
      第68回土木学会年次学術講演会
    • Place of Presentation
      大阪
    • Year and Date
      2014-09-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] 下水処理UASBプロセスのグラニュール汚泥内に存在する主要未培養微生物の空間分布と機能推定2013

    • Author(s)
      井口晃徳, 千葉有紀, 林真由美, 山口隆司, 原田秀樹, 重松亨
    • Organizer
      第29回日本微生物生態学会大会
    • Place of Presentation
      鹿児島、日本
    • Related Report
      2013 Research-status Report
  • [Presentation] Microbial community structure of practical-scale Down-flow Hanging Sponge reactor treating municipal wastewater in India.2013

    • Author(s)
      Akinori IGUCHI, Tsutomu Okubo, Masanobu Takahashi, Masashi Hatamoto, Takashi Yamaguchi, Shigeki Uemura, Kengo Kubota and Hideki Harada.
    • Organizer
      The 1st International Forum On Asian Water Environment Technology
    • Place of Presentation
      Delhi, India
    • Related Report
      2013 Research-status Report
  • [Presentation] 下水処理メタン発酵プロセスにおいて高頻度に検出される未培養微生物の集積化と機能推定2013

    • Author(s)
      井口晃徳, 千葉有紀, 林真由美, 山口隆司, 原田秀樹, 重松亨.
    • Organizer
      第47回日本水環境学会年会
    • Place of Presentation
      大阪工業大学 (大阪市)
    • Related Report
      2012 Research-status Report
  • [Presentation] Microbial community and functions of low-strength methane fermentation process with no temperature control.2012

    • Author(s)
      Akinori IGUCHI, Tsutomu Okubo, Masanobu Takahashi, Kengo Kubota, Takashi Yamaguchi, Yuji Sekiguchi, Toru Shigematsu, Nobuo Araki, Hideki Harada.
    • Organizer
      28th Annual Meeting of the Japanese Society of Microbial Ecology (JSME2012)
    • Place of Presentation
      豊橋技術科学大学 (豊橋市)
    • Related Report
      2012 Research-status Report
  • [Presentation] 都市下水処理メタン発酵プロセスにおいて高頻度に検出される未培養微生物の視覚化と定量2012

    • Author(s)
      井口晃徳, 千葉有紀, 林真由美, 山口隆司, 原田秀樹, 重松亨.
    • Organizer
      日本農芸化学会2012年度関東支部大会
    • Place of Presentation
      新潟薬科大学 (新潟市)
    • Related Report
      2012 Research-status Report
  • [Presentation] 下水処理メタン発酵プロセスにおいて高頻度に検出される未培養微生物の機能解析2012

    • Author(s)
      井口晃徳
    • Organizer
      第2回 バイオマス&エネルギー expo in 新潟
    • Place of Presentation
      朱鷺メッセ (新潟市)
    • Related Report
      2012 Research-status Report
  • [Remarks] 新潟薬科大学 産官学連携推進センター

    • URL

      http://www.nupals.ac.jp/liaison/news/2012/12/expo.html

    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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