Development of novel high sensitive FISH for revealing the microbial ecology of uncultured microorganisms
Project/Area Number |
24760437
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Civil and environmental engineering
|
Research Institution | Anan National College of Technology |
Principal Investigator |
KAWAKAMI Shuji 阿南工業高等専門学校, その他部局等, 助教 (00610461)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 高感度FISH法 / シングルセル解析 / 環境微生物学 / 環境微生物 / バイオリクター / FISH法 / in situ HCR法 |
Outline of Final Research Achievements |
In this study, we present a non-enzymatic, hybridization chain reaction (HCR)-based signal amplified in situ whole-cell detection technique (in situ DNA-HCR), and aptamer-based live-cell detection technique for revealing the microbial ecology of uncultured microorganisms. They are a simple and easy technique for detecting single microbial cells and enhancing understanding of the ecology and behaviour of environmental microorganisms in situ.
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Report
(4 results)
Research Products
(18 results)