| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
The specificity of quantitative polymerase chain reaction (qPCR) assays targeting geosmin synthase gene was evaluated using cyanobacterial strains isolated form domestic water sources. Then selected high specific qPCR assay was applied to analyze water samples obtained from one water reservoir where geosmin producing cyanobacteria often occurs.
The geosmin synthase gene was successfully detected even when geosmin concentration was low. In addition, the number of geosmin synthase genes analyzed by the qPCR was well correlated with geosmin concentration in the water reservoir. These results demonstrated that the qPCR assay can be used as an early, easy and quantitative detection method of geosmin-producing cyanobacteria in drinking water sources.
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