| Project/Area Number |
24760647
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Tokyo University of Technology (2013)
Tokyo University of Agriculture and Technology (2012) |
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Principal Investigator |
YOSHIDA Wataru 東京工科大学, 応用生物学部, 助教 (10599806)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | アプタマー / in vitro selection / メチル化DNA / ＤＮＡ methylation / Ａｐｔａｍｅｒ |
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| Research Abstract |
The research project aimed to identify RNA aptamer that specifically recognizes methylated DNA and develop high sensitive detection system for DNA methylation on target gene. To identify the aptamer, in vitro selection was performed against methylated duplex DNA. After 5th round selection, the RNA library was enriched. By sequencing analysis of the enriched RNA library, four kinds of sequence were identified in the library. The RNAs were synthesized and then binding assay against methylated DNA was performed. As the results, the RNAs bound to both of the methylated duplex DNA and unmethylated duplex DNA with 10 nM Kd. Therefore, it is expected that DNA methylation-binding RNA aptamer might be obtained by improving the binding specificity of the identified RNA aptamer and it might contribute for development of the high sensitive DNA methylation detection system.
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