• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of novel culture technique for neural stem cells using polyacrylic acid-grafted fabrics

Research Project

Project/Area Number 24760652
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biofunction/Bioprocess
Research InstitutionOsaka Prefecture University

Principal Investigator

MORI Hideki  大阪府立大学, 理学(系)研究科(研究院), 助教 (30450894)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsneural stem cell / Nylon / fabric / cell culture / spheroid / 細胞培養 / 神経幹細胞 / 高分子繊維 / 放射線 / グラフト重合 / stem cell / ポリアミド
Outline of Final Research Achievements

Acrylic acid (AAc) was grafted onto Nylon (PA66) fabrics through graft polymerization induced by gamma-ray pre-irradiation. Activation of the carboxyl groups in poly AAc (PAAc) induced by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)/ N-hydroxyscuccinimide (NHS) treatment allowed covalent immobilization of proteins (Matrigel, etc.). The effects of the PAAc-graftred and protein-immobilized fabrics on the adherence and differentiation of mouse neural stem/progenitor cells (NSPCs) were investigated. Matrigel-immobilized PA66 fabric was the best for the growth of NSPCs in the experimental fabrics.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (9 results)

All 2014 2013 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (5 results) Remarks (2 results)

  • [Journal Article] Influence of amino acids, buffers, and pH on the γ-irradiation-induced degradation of alginates2014

    • Author(s)
      A.-S. T. Ulset, H. Mori, M. Ø. Dalheim, M. Hara, B. E. Christensen
    • Journal Title

      Biomacromolecules

      Volume: 15 Issue: 12 Pages: 4590-4597

    • DOI

      10.1021/bm501386n

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic viscoelastic properties of collagen gels with high mechanical strength2013

    • Author(s)
      Hideki Mori, Kousuke Shimizu, Masayuki Hara
    • Journal Title

      Materials Science and Engineering C

      Volume: 33 Pages: 3230-3236

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 網目状ナイロン繊維に対する神経幹細胞/前駆細胞の接着性評価2014

    • Author(s)
      中亮介、森英樹、原正之
    • Organizer
      第36回日本バイオマテリアル学会大会
    • Place of Presentation
      タワーホール船堀 (東京都)
    • Year and Date
      2014-11-17 – 2014-11-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] ポリアクリル酸修飾ナイロン繊維に対する神経幹細胞/前駆細胞の接着性の評価2014

    • Author(s)
      中亮介、森英樹、原正之
    • Organizer
      日本バイオマテリアル学会第9回関西若手研究発表会
    • Place of Presentation
      京都大学再生医科学研究所 (京都市)
    • Year and Date
      2014-08-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] 力学的特性の異なるガンマ線架橋ポリビニルアルコールゲル上における神経幹細胞/前駆細胞の培養2014

    • Author(s)
      森英樹・太田愛里・原正之
    • Organizer
      日本化学会第94春季大会
    • Place of Presentation
      名古屋大学 東山キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] 放射線架橋ポリビニルアルコールゲルを用いた神経幹細胞/前駆細胞の培養2013

    • Author(s)
      森英樹、川瀬文音、原正之
    • Organizer
      第65回日本生物工学会大会
    • Place of Presentation
      広島国際会議場
    • Related Report
      2013 Research-status Report
  • [Presentation] ガンマ線架橋ポリビニルアルコールゲル上における神経幹細胞/前駆細胞の培養2013

    • Author(s)
      森 英樹
    • Organizer
      日本化学会第93春季年会
    • Place of Presentation
      立命館大学びわこ・くさつキャンパス(滋賀県)
    • Related Report
      2012 Research-status Report
  • [Remarks] 細胞組織工学分野のホームページ

    • URL

      http://www.b.s.osakafu-u.ac.jp/~hara/index.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 大阪府立大学 大学院理学系研究科生物科学専攻 細胞組織工学分野

    • URL

      http://www.b.s.osakafu-u.ac.jp/~hara/index.html

    • Related Report
      2013 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi