| Project/Area Number |
24760655
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | National Institute for Materials Science |
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Principal Investigator |
YAMAZAKI TOMOHIKO 独立行政法人物質・材料研究機構, 国際ナノアーキテクトニクス研究拠点, MANA研究者 (50419264)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | トール様受容体 / 合成オリゴデオキシヌクレオチド / アレルギー / DNA結合部位 / TLR9 / CpG ODNs / トール様受容体9 (TLR9) / オリゴデオキシヌクレオチド / CpG ODN / トール様受容体9 / 合成オリゴデオキシリボヌクレオチド / DNA結合 |
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| Research Abstract |
Unmethylated cytosine-guanine (CpG) motif-containing oligodeoxynucleotides (ODNs) had been enormously studied for its adjuvant-potential to stimulate innate immune response via interaction with the pattern-recognition receptor Toll-like receptor 9 (TLR9). Activation of TLR9 by CpG ODN induces a signal transduction cascade that plays a pivotal role in first-line immune defense in the human body. The three-dimensional structure of TLR9 has not yet been reported, and the ligand-binding mechanism of TLR9 is still poorly understood; therefore, the mechanism of human TLR9 (hTLR9) ligand binding needs to be elucidated. In this study, we showed that H505, H530, and Y554 were vicinal-oriented and formed positively charged clusters with which negatively charged ODN could interact.
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