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Construction of tolerant yeast of fermentation inhibitors for ethanol production from lignocellulosic biomass

Research Project

Project/Area Number 24760730
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Energy engineering
Research InstitutionHiroshima University

Principal Investigator

KANNA Machi  広島大学, 工学(系)研究科(研究院), 助教 (10619365)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords発酵阻害物質 / エタノール発酵 / バイオマス / 酵母 / 国際情報交換
Research Abstract

Lignocellulosic biomass passes through the process of three steps: pretreatment, enzymatic hydrolysis and ethanol fermentation. During the process of hydrothermal pretreatment that is one of the pretreatment, fermentation inhibitors are generated from lignocellulosic biomass. In this study, we analyzed quantification of various fermentation inhibitors derived from hydrothermal pretreatment. Furthermore, we investigated an effect of fermentation inhibitors on yeast growth and fermentation. As a result, furfural, 5-HMF and formic acid affected both yeast growth and ethanol fermentation under the same condition. However, in the case of acetic acid, inhibition of fermentation was not caused by high concentration though the rate of yeast growth decreased at the same condition. Moreover, we determined the effects of inhibition substance on yeast growth by fitting to Monod equation. Also, we quantified the effect of these inhibitors on the Monod parameters.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (13 results)

All 2013 2012

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (11 results)

  • [Journal Article] 発酵阻害物質が酵母増殖のMonod式に与える影響2013

    • Author(s)
      神名麻智, 木村直人, 山下康貴, 柳田高志, 松村幸彦
    • Journal Title

      J.Jpn.Petrol.Inst.

      Volume: 56 Pages: 326-330

    • NAID

      130003383575

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 発酵阻害物質が酵母増殖のMonod式に与える影響2013

    • Author(s)
      神名麻智, 木村直人, 山下康貴, 柳田高志, 松村幸彦
    • Journal Title

      J. Jpn. Petrol. Inst.

      Volume: 56 Pages: 326-330

    • NAID

      130003383575

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] Effect of inhibitors from hydrothermal pretreatment on ethanol fermentation2013

    • Author(s)
      Fukutomi Yuta, Yanagida Takashi, Kanna Machi., Matsumura Yukihiko
    • Organizer
      20^<th> Europian Biomass Conference & Exhibition
    • Place of Presentation
      Copenhagen Denmark
    • Related Report
      2013 Final Research Report
  • [Presentation] Effect of fermentation inhibitors derived from hydrothermal pretreatment on yeast2013

    • Author(s)
      Kanna Machi, Fukutomi Yuta, Yamashita Kouki, Yanagida Takashi Matsumura Yukihiko
    • Organizer
      9^<th> Biomass-Asia Workshop
    • Place of Presentation
      Tokyo Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] 酵阻害物質と温度が酵母増殖に及ぼす影響2013

    • Author(s)
      神名麻智, 山下康貴, 福富裕太, 松村幸彦
    • Organizer
      日本機械学会
    • Place of Presentation
      岡山大学
    • Related Report
      2013 Final Research Report
  • [Presentation] 発酵阻害物質が発酵におよぼす影響の定量的解析2013

    • Author(s)
      神名麻智, 福富裕太, 柳田高志, 松村幸彦
    • Organizer
      化学工学会
    • Place of Presentation
      岡山大学
    • Related Report
      2013 Final Research Report
  • [Presentation] Effect of inhibitors from hydrothermal pretreatment on ethanol fermentation2013

    • Author(s)
      Fukutomi, Y., Yanagida, T., Kanna, M., Matsumura, Y.
    • Organizer
      20th European Biomass Conference & Exhibition
    • Related Report
      2013 Annual Research Report
  • [Presentation] Effect of fermentation inhibitors derived from hydrothermal pretreatment on yeast2013

    • Author(s)
      Kanna, M., Fukutomi, Y., Yamashita, K., Yanagida, T., Matsumura, Y.
    • Organizer
      9th Biomass-Asia Workshop
    • Related Report
      2013 Annual Research Report
  • [Presentation] 発酵阻害物質と温度が酵母増殖に及ぼす影響2013

    • Author(s)
      神名麻智,山下康貴,福富裕太,松村幸彦
    • Organizer
      日本機械学会
    • Related Report
      2013 Annual Research Report
  • [Presentation] 発酵阻害物質が発酵に及ぼす影響の定量的解析2013

    • Author(s)
      神名麻智,福富裕太,柳田高志,松村幸彦
    • Organizer
      化学工学会
    • Related Report
      2013 Annual Research Report
  • [Presentation] 水熱前処理によって生成する発酵阻害物質の酵母に対する影響2012

    • Author(s)
      神名麻智、木村直人、山下康貴、柳田高志、松村幸彦
    • Organizer
      日本エネルギー学会
    • Place of Presentation
      東京都
    • Related Report
      2012 Research-status Report
  • [Presentation] Effect of fermentation inhibitors derived from hydrothermal pretreatment on yeast2012

    • Author(s)
      Machi Kanna, Yuta Fukutomi, Kouki Yamashita, Takashi Yanagida, Yukihiko Matsumura
    • Organizer
      バイオマスアジアワークショップ
    • Place of Presentation
      東京都
    • Related Report
      2012 Research-status Report
  • [Presentation] 酵母の挙動に及ぼす水熱前処理副生成物質の影響2012

    • Author(s)
      神名麻智、山下康貴、福富裕太、松村幸彦
    • Organizer
      化学工学会
    • Place of Presentation
      仙台
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-12-27  

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