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Initiation of nuclear divisions and restoration of diploidy in parthenogenetic embryos of Drosophila ananassae

Research Project

Project/Area Number 24770006
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Genetics/Genome dynamics
Research InstitutionKyorin University

Principal Investigator

HIRAI Kazuyuki  杏林大学, 医学部, 講師 (70597335)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords遺伝学 / 二倍体 / 中心体 / 受精 / 単為発生 / ショウジョウバエ / ゲノム / 細胞周期 / 単為生殖 / 染色体 / 減数分裂
Outline of Final Research Achievements

Parthenogenesis is the development of an unfertilized egg into a new individual. We here examined the mechanisms by which the following problems are solved in parthenogenetic strains of Drosophila ananassae: Mitotic division of the female pronucleus and restoration of diploidy. We found that, in unfertilized eggs of both sexual and parthenogenetic strains, an acentrosomal spindle is assembled around the chromosomes of the female pronucleus, after completion of S phase. It is striking that eggs that were laid by parthenogenetic females assembled centrosome-like structures de novo, which were scattered in the anterior part of the egg cytoplasm. Our data suggest a role of the centrosome-like structures in stimulating the nuclear division of the female pronucleus. We also found that the first diploid nucleus is formed by fusion of two haploid chromosome sets at telophase of the second nuclear cycle of parthenogenetic embryos, resembling the gonomeric division in sexual development.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (9 results)

All 2014 2013 2012

All Presentation (9 results) (of which Invited: 1 results)

  • [Presentation] De novo synthesis of centrosome-like structures and diploidization in partheogenetic embryos of Drosophila ananassae2014

    • Author(s)
      Kazuyuki Hirai, Yukio Shima, Yohei Minakuchi, Atsushi Toyoda, Muneo Matsuda
    • Organizer
      Cold Spring Harbor Laboratory meeting on Germ Cells
    • Place of Presentation
      Cold Spring Harbor Laboratory、米国ニューヨーク州
    • Year and Date
      2014-10-07 – 2014-10-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] De novo centrosome synthesis and diploidization in partheogenetic embryos of Drosophila ananassae2014

    • Author(s)
      平井和之、島幸夫、水口洋平、豊田敦、松田宗男
    • Organizer
      日本遺伝学会第86回大会
    • Place of Presentation
      滋賀県、長浜バイオ大学
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] De novo centrosome synthesis and diploidization in parthenogenetic embryos of Drosophila ananassae-complex species2014

    • Author(s)
      Kazuyuki Hirai, Akihiko Kudo, Yukio Shima, Yohei Minakuchi, Atsushi Toyoda, Muneo Matsuda
    • Organizer
      The 11th Japanese Drosophila Research Conference
    • Place of Presentation
      石川県、金沢歌劇座
    • Year and Date
      2014-06-04 – 2014-06-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] de novo centrosome synthesis in parthenogenetic embryos of Drosophila ananassae-complex species2013

    • Author(s)
      平井和之、宮東昭彦、松田宗男
    • Organizer
      第65回日本細胞生物学会大会
    • Place of Presentation
      名古屋市
    • Related Report
      2013 Research-status Report 2012 Research-status Report
  • [Presentation] De novo synthesis of centrosomes and diploidization in parthenogenetic embryos of Drosophila ananassae2013

    • Author(s)
      Kazuyuki Hirai, Akihiko Kudo, and Muneo Matsuda
    • Organizer
      EMBO workshop Drosophila cell division cycle
    • Place of Presentation
      トットネス、英国
    • Related Report
      2013 Research-status Report
  • [Presentation] アナナスショウジョウバエにおける単為発生開始機構の解析2013

    • Author(s)
      平井和之、松田宗男
    • Organizer
      国立遺伝学研究所共同研究会、ショウジョウバエ多様性研究会
    • Place of Presentation
      三島市
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] De novo centrosome synthesis and diploidization in parthenogenetic embryos of Drosophila ananassae-complex species2013

    • Author(s)
      Kazuyuki Hirai, Muneo Matsuda
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸市
    • Related Report
      2013 Research-status Report
  • [Presentation] アナナスショウジョウバエ単為発生系統における2倍体化機構の解析2012

    • Author(s)
      平井和之、宮東昭彦、松田宗男
    • Organizer
      日本遺伝学会第84回大会
    • Place of Presentation
      福岡市
    • Related Report
      2012 Research-status Report
  • [Presentation] Cellular basis of diploidization in Drosophila ananassae parthenogenesis2012

    • Author(s)
      Kazuyuki Hirai, Akihiko Kudo, and Muneo Matsuda
    • Organizer
      Germ Cells
    • Place of Presentation
      Cold Spring Harbor、米国
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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