Soil microbial community analysis to identify syntrophic relationships between microbes
| Project/Area Number |
24770015
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ecology/Environment
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
MORI Hiroshi 東京工業大学, 生命理工学研究科, 助教 (40610837)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 細菌群集 / 代謝 / メタゲノム / バイオインフォマティクス / 生態 |
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| Outline of Final Research Achievements |
We conducted a bioinformatics analysis of a time-series metagenomic sequencing data of a soil microbial community with the aromatic compounds pollution. Developing and using the bioinformatics method to efficiently identify fluctuating genes and taxa in time-series metagenomic data, we identified a catalog of fluctuating taxa and genes during aromatic compounds degradation in the soil. In addition, we developed a tool VITCOMIC2 which can (i) identify 16S rRNA gene sequences in metagenome sequence data with minimum false positive rate, (ii) assign taxonomy of the sequences, and (iii) calculate taxonomic composition of the sample.
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Report
(4 results)
Research Products
(12 results)
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[Presentation] Design and experimental validation of a novel non-degenerate universal primer set for 16S rRNA gene amplicon sequencing with a low possibility to amplify eukaryotic rRNA genes2013
Author(s)
Hiroshi Mori, Fumito Maruyama, Hiromi Kato, Atsushi Toyoda, Ayumi Dozono, Yoshiyuki Ohtsubo, Yuji Nagata, Asao Fujiyama, Masataka Tsuda, and Ken Kurokawa
Organizer
2nd Thunen Symposium on Soil Metagenomics
Place of Presentation
Thunen Institute, Braunschweig, Germany
Related Report
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